ArticlePloS one2013
A method for 3D immunostaining and optical imaging of the mouse brain demonstrated in neural progenitor cells.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 39 citations in OpenAlex.
- Uniform volumetric single-cell processing for organ-scale molecular phenotyping.Nature biotechnology · 2025Article
- Optical imaging of the small intestine immune compartment across scales.Communications biology · 2023Article
- Nanotheranostic Strategies for Cancer Immunotherapy.Small methods · 2022Review
- Tutorial: practical considerations for tissue clearing and imaging.Nature protocols · 2021Review
- Whole Murine Brain Imaging Based on Optical Elastic Scattering.Advances in experimental medicine and biology · 2021Article
- TISSUE CLEARING.Nature reviews. Methods primers · 2021Article
- High-Throughput Strategy for Profiling Sequential Section With Multiplex Staining of Mouse Brain.Frontiers in neuroanatomy · 2021Article
- Comparison of Different Tissue Clearing Methods for Three-Dimensional Reconstruction of Human Brain Cellular Anatomy Using Advanced Imaging Techniques.Frontiers in neuroanatomy · 2021Article
- Whole-Brain Profiling of Cells and Circuits in Mammals by Tissue Clearing and Light-Sheet Microscopy.Neuron · 2020Review
- Versatile whole-organ/body staining and imaging based on electrolyte-gel properties of biological tissues.Nature communications · 2020Article
- Three-dimensional visualization of intramuscular innervation in intact adult skeletal muscle by a modified iDISCO method.Neurophotonics · 2020Article
- Pathogenesis of Alzheimer's Disease Examined Using a Modified Puri-Li Model that Incorporates Calcium Ion Homeostasis.Journal of molecular neuroscience : MN · 2018Article
- 3D chemical imaging of the brain using quantitative IR spectro-microscopy.Chemical science · 2018Article
- Optical projection tomography for rapid whole mouse brain imaging.Biomedical optics express · 2017Article
- 3D visualization and quantification of microvessels in the whole ischemic mouse brain using solvent-based clearing and light sheet microscopy.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2017Article
- Examining the Role of Microglia and Astroglia During the Pathogenesis of Alzheimer's Disease via the Puri-Li Model.Journal of molecular neuroscience : MN · 2017Article
- Molecular imaging of biological systems with a clickable dye in the broad 800- to 1,700-nm near-infrared window.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- A Whole Brain Staining, Embedding, and Clearing Pipeline for Adult Zebrafish to Visualize Cell Proliferation and Morphology in 3-Dimensions.Frontiers in neuroscience · 2017Article
- DALMATIAN: An Algorithm for Automatic Cell Detection and Counting in 3D.Frontiers in neuroanatomy · 2017Article
- Clearing and Labeling Techniques for Large-Scale Biological Tissues.Molecules and cells · 2016Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It is important to understand changes in cell distribution that occur as a part of disease progression. This is typically achieved using standard sectioning and immunostaining, however, many structures and cell distribution patterns are not readily appreciated in two-dimensions, including the distribution of neural stem and progenitor cells in the mouse forebrain. Three-dimensional immunostaining in the mouse brain has been hampered by poor penetration. For this reason, we have developed a method that allows for entire hemispheres of the mouse brain to be stained using commercially available antibodies. Brains stained for glial fibrillary acidic protein, doublecortin and nestin were imaged in three-dimensions using optical projection tomography and serial two-photon tomography. This staining method is simple, using a combination of heat, time and specimen preparation procedures readily available, so that it can be easily implemented without the need for specialized equipment, making it accessible to most laboratories.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.