ArticlePloS one2013
Geometric attributes of retaining glycosyltransferase enzymes favor an orthogonal mechanism.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- NonglycosidicSynthetic and systems biotechnology · 2025Article
- A substrate-multiplexed platform for profiling enzymatic potential of plant family 1 glycosyltransferases.Nature communications · 2025Article
- Structural insights into terminal arabinosylation of mycobacterial cell wall arabinan.Nature communications · 2025Article
- Structure-Based Mechanism and Specificity of Human Galactosyltransferase β3GalT5.Journal of the American Chemical Society · 2025Article
- An improved expression and purification protocol enables the structural characterization of Mnt1, an antifungal target from Candida albicans.Fungal biology and biotechnology · 2024Article
- Novel Soybean Variety Lacking Raffinose Synthase 2 Activity.ACS omega · 2024Article
- Speaking the host language: howMicrobiology (Reading, England) · 2023Review
- Structure of the human heparan sulfate polymerase complex EXT1-EXT2.Nature communications · 2022Article
- Xyloglucan Biosynthesis: From Genes to Proteins and Their Functions.Frontiers in plant science · 2022Review
- Polysaccharide Biosynthesis: Glycosyltransferases and Their Complexes.Frontiers in plant science · 2021Article
- Type III Secretion Effectors with Arginine N-Glycosyltransferase Activity.Microorganisms · 2020Review
- Emerging structural insights into glycosyltransferase-mediated synthesis of glycans.Nature chemical biology · 2019Review
- Structural basis for arginine glycosylation of host substrates by bacterial effector proteins.Nature communications · 2018Article
- Strategies toward protecting group-free glycosylation through selective activation of the anomeric center.Beilstein journal of organic chemistry · 2017Review
- Bacterial Glycosyltransferases: Challenges and Opportunities of a Highly Diverse Enzyme Class Toward Tailoring Natural Products.Frontiers in microbiology · 2016Article
- Notch-modifying xylosyltransferase structures support an SNi-like retaining mechanism.Nature chemical biology · 2015Article
- A computational and experimental study of O-glycosylation. Catalysis by human UDP-GalNAc polypeptide:GalNAc transferase-T2.Organic & biomolecular chemistry · 2014Article
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Authors and funding
3 authors.
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Abstract
Retaining glycosyltransferase enzymes retain the stereochemistry of the donor glycosidic linkage after transfer to an acceptor molecule. The mechanism these enzymes utilize to achieve retention of the anomeric stereochemistry has been a matter of much debate. Re-analysis of previously released structural data from retaining and inverting glycosyltransferases allows competing mechanistic proposals to be evaluated. The binding of metal-nucleotide-sugars between inverting and retaining enzymes is conformationally unique and requires the donor substrate to occupy two different orientations in the two types of glycosyltransferases. The available structures of retaining glycosyltransferases lack appropriately positioned enzymatic dipolar residues to initiate or stabilize the intermediates of a dissociative mechanism. Further, available structures show that the acceptor nucleophile and anomeric carbon of the donor sugar are in close proximity. Structural features support orthogonal (front-side) attack from a position lying ≤ 90° from the C1-O phosphate bond for retaining enzymes. These structural conclusions are consistent with the geometric conclusions of recent kinetic and computational studies.
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