ArticlePloS one2013
A R2R3-MYB transcription factor from Epimedium sagittatum regulates the flavonoid biosynthetic pathway.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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48 citing papers in PubMed, 85 citations in OpenAlex.
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- ZmMYB155 is involved in starch synthesis and basal endosperm transfer layer development in maize.Plant cell reports · 2025Article
- Resequencing and Functional Analysis Revealed ThatInternational journal of molecular sciences · 2025Article
- Boosting transcriptional activities by employing repeated activation domains in transcription factors.The Plant cell · 2025Article
- Integrated metabolomic and transcriptomic analysis reveals biosynthesis mechanism of flavone and caffeoylquinic acid in chrysanthemum.BMC genomics · 2024Article
- Identification and Expression Analysis of R2R3-MYB Transcription Factors Associated with Flavonoid Biosynthesis inInternational journal of molecular sciences · 2024Article
- Cloned genes and genetic regulation of anthocyanin biosynthesis in maize, a comparative review.Frontiers in plant science · 2024Review
- Multilayered regulation of secondary metabolism in medicinal plants.Molecular horticulture · 2023Review
- Phylogenetic Analysis ofBiomolecules · 2023Article
- Time-series transcriptome provides insights into the gene regulation network involved in the icariin-flavonoid metabolism during the leaf development ofFrontiers in plant science · 2023Article
- Genetic and molecular analysis of the anthocyanin pigmentation pathway inFrontiers in plant science · 2023Article
- MYB transcription factors and their role in Medicinal plants.Molecular biology reports · 2022Review
- Glucose Supply Induces PsMYB2-Mediated Anthocyanin Accumulation inFrontiers in plant science · 2022Article
- Comparative Transcriptomic Analysis on White and Blue Flowers of Platycodon grandiflorus to Elucidate Genes Involved in the Biosynthesis of Anthocyanins.Iranian journal of biotechnology · 2021Article
- The methyl jasmonate-responsive transcription factor SmMYB1 promotes phenolic acid biosynthesis in Salvia miltiorrhiza.Horticulture research · 2021Article
- Alternative Splicing Dynamics During the Lifecycle ofFrontiers in plant science · 2021Article
- Cytosolic TaGAPC2 Enhances Tolerance to Drought Stress in Transgenic Arabidopsis Plants.International journal of molecular sciences · 2020Article
- Transcriptome and metabolome analyses reveal the regulation of peel coloration in green, red Chinese prickly ash (Food chemistry. Molecular sciences · 2020Article
- De novo transcriptome sequencing of Rhododendron molle and identification of genes involved in the biosynthesis of secondary metabolites.BMC plant biology · 2020Article
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8 authors at 5 institutions in 2 countries.
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No grant is acknowledged in the PubMed record.
Abstract
Herba epimedii (Epimedium), a traditional Chinese medicine, has been widely used as a kidney tonic and antirheumatic medicine for thousands of years. The bioactive components in herba epimedii are mainly prenylated flavonol glycosides, end-products of the flavonoid pathway. Epimedium species are also used as garden plants due to the colorful flowers and leaves. Many R2R3-MYB transcription factors (TFs) have been identified to regulate the flavonoid and anthocyanin biosynthetic pathways. However, little is known about the R2R3-MYB TFs involved in regulation of the flavonoid pathway in Epimedium. Here, we reported the isolation and functional characterization of the first R2R3-MYB TF (EsMYBA1) from Epimedium sagittatum (Sieb. Et Zucc.) Maxim. Conserved domains and phylogenetic analysis showed that EsMYBA1 belonged to the subgroup 6 clade (anthocyanin-related MYB clade) of R2R3-MYB family, which includes Arabidopsis AtPAP1, apple MdMYB10 and legume MtLAP1. EsMYBA1 was preferentially expressed in leaves, especially in red leaves that contain higher content of anthocyanin. Alternative splicing of EsMYBA1 resulted in three transcripts and two of them encoded a MYB-related protein. Yeast two-hybrid and transient luciferase expression assay showed that EsMYBA1 can interact with several bHLH regulators of the flavonoid pathway and activate the promoters of dihydroflavonol 4-reductase (DFR) and anthocyanidin synthase (ANS). In both transgenic tobacco and Arabidopsis, overexpression of EsMYBA1 induced strong anthocyanin accumulation in reproductive and/or vegetative tissues via up-regulation of the main flavonoid-related genes. Furthermore, transient expression of EsMYBA1 in E. sagittatum leaves by Agrobacterium infiltration also induced anthocyanin accumulation in the wounded area. This first functional characterization of R2R3-MYB TFs in Epimedium species will promote further studies of the flavonoid biosynthesis and regulation in medicinal plants.
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