ArticlePloS one2013
Inter- and intra-host viral diversity in a large seasonal DENV2 outbreak.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 73 citations in OpenAlex.
- Whole Genome Sequencing of DENV-2 isolated frombioRxiv : the preprint server for biology · 2024Article
- Detection of coinfection with Primate Erythroparvovirus 1 and arboviruses (DENV, CHIKV and ZIKV) in individuals with acute febrile illness in the state of Rio Grande do Norte in 2016.PLoS neglected tropical diseases · 2023Article
- Intracellular Diversity of WNV within Circulating Avian Peripheral Blood Mononuclear Cells Reveals Host-Dependent Patterns of Polyinfection.Pathogens (Basel, Switzerland) · 2023Article
- Transgene-induced cell death following dengue-2 virus infection in Aedes aegypti.Scientific reports · 2023Article
- Intracellular diversity of WNV within circulating avian peripheral blood mononuclear cells reveals host-dependent patterns of polyinfection.bioRxiv : the preprint server for biology · 2023Article
- Contribution of phylogenetics to understanding the evolution and epidemiology of dengue virus.Animal models and experimental medicine · 2022Review
- Spatio-temporal dynamics of intra-host variability in SARS-CoV-2 genomes.Nucleic acids research · 2022Article
- Application of Next-Generation Sequencing to Reveal How Evolutionary Dynamics of Viral Population Shape Dengue Epidemiology.Frontiers in microbiology · 2020Review
- Transgenic Aedes aegypti Mosquitoes Transfer Genes into a Natural Population.Scientific reports · 2019Article
- Endless Forms: Within-Host Variation in the Structure of the West Nile Virus RNA Genome during Serial Passage in Bird Hosts.mSphere · 2019Article
- Tracking dengue virus type 1 genetic diversity during lineage replacement in an hyperendemic area in Colombia.PloS one · 2019Article
- Inter- and intra-host sequence diversity reveal the emergence of viral variants during an overwintering epidemic caused by dengue virus serotype 2 in southern Taiwan.PLoS neglected tropical diseases · 2018Article
- Understanding dengue virus evolution to support epidemic surveillance and counter-measure development.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2018Review
- Article
- Increasing Clinical Severity during a Dengue Virus Type 3 Cuban Epidemic: Deep Sequencing of Evolving Viral Populations.Journal of virology · 2016Article
- Intra-epidemic evolutionary dynamics of a Dengue virus type 1 population reveal mutant spectra that correlate with disease transmission.Scientific reports · 2016Article
- Genomic approaches for understanding dengue: insights from the virus, vector, and host.Genome biology · 2016Review
- West Nile Virus Population Structure, Injury, and Interferon-Stimulated Gene Expression in the Brain From a Fatal Case of Encephalitis.Open forum infectious diseases · 2016Article
- Complete Genome Sequence of an Atypical Dengue Virus Serotype 2 Lineage Isolated in Brazil.Genome announcements · 2015Article
- Error correction and statistical analyses for intra-host comparisons of feline immunodeficiency virus diversity from high-throughput sequencing data.BMC bioinformatics · 2015Article
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 3 countries.
Funding
Abstract
backgroundHigh genetic diversity at both inter- and intra-host level are hallmarks of RNA viruses due to the error-prone nature of their genome replication. Several groups have evaluated the extent of viral variability using different RNA virus deep sequencing methods. Although much of this effort has been dedicated to pathogens that cause chronic infections in humans, few studies investigated arthropod-borne, acute viral infections. METHODS AND PRINCIPAL
findingsWe deep sequenced the complete genome of ten DENV2 isolates from representative classical and severe cases sampled in a large outbreak in Brazil using two different approaches. Analysis of the consensus genomes confirmed the larger extent of the 2010 epidemic in comparison to a previous epidemic caused by the same viruses in another city two years before (genetic distance = 0.002 and 0.0008 respectively). Analysis of viral populations within the host revealed a high level of conservation. After excluding homopolymer regions of 454/Roche generated sequences, we found 10 to 44 variable sites per genome population at a frequency of >1%, resulting in very low intra-host genetic diversity. While up to 60% of all variable sites at intra-host level were non-synonymous changes, only 10% of inter-host variability resulted from non-synonymous mutations, indicative of purifying selection at the population level. CONCLUSIONS AND SIGNIFICANCE: Despite the error-prone nature of RNA-dependent RNA-polymerase, dengue viruses maintain low levels of intra-host variability.
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