ArticlePloS one2013
A role for the protein tyrosine phosphatase CD45 in macrophage adhesion through the regulation of paxillin degradation.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 28 citations in OpenAlex.
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- Paxillin and Kindlin: Research Progress and Biological Functions.Biomolecules · 2025Review
- Comprehensive proteomic analysis of the differential expression of 62 proteins following intracortical microelectrode implantation.Scientific reports · 2024Article
- Single-nucleus RNA sequencing and lipidomics reveal characteristics of transcriptional and lipid composition in porcine longissimus dorsi muscle.BMC genomics · 2024Article
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- Comparison of the Toponomes of Alveolar Macrophages From Wild Type and Surfactant Protein A Knockout Mice and Their Response to Infection.Frontiers in immunology · 2022Article
- Fatty acid nitroalkenes inhibit the inflammatory response to bleomycin-mediated lung injury.Toxicology and applied pharmacology · 2020Article
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- Apoptotic tumor cell-derived microRNA-375 uses CD36 to alter the tumor-associated macrophage phenotype.Nature communications · 2019Article
- Influenza Virus Infections and Cellular Kinases.Viruses · 2019Review
- Extracellular Vesicles Shed ByFrontiers in immunology · 2018Article
- The macrophage cytoskeleton acts as a contact sensor upon interaction with Entamoeba histolytica to trigger IL-1β secretion.PLoS pathogens · 2017Article
- The Mouse Cytomegalovirus Gene m42 Targets Surface Expression of the Protein Tyrosine Phosphatase CD45 in Infected Macrophages.PLoS pathogens · 2016Article
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- Cytomegalovirus immune evasion of myeloid lineage cells.Medical microbiology and immunology · 2015Review
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Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CD45 is a protein tyrosine phosphatase expressed on all cells of hematopoietic origin that is known to regulate Src family kinases. In macrophages, the absence of CD45 has been linked to defects in adhesion, however the molecular mechanisms involved remain poorly defined. In this study, we show that bone marrow derived macrophages from CD45-deficient mice exhibit abnormal cell morphology and defective motility. These defects are accompanied by substantially decreased levels of the cytoskeletal-associated protein paxillin, without affecting the levels of other proteins. Degradation of paxillin in CD45-deficient macrophages is calpain-mediated, as treatment with a calpain inhibitor restores paxillin levels in these cells and enhances cell spreading. Inhibition of the tyrosine kinases proline-rich tyrosine kinase (Pyk2) and focal adhesion kinase (FAK), kinases that are capable of mediating tyrosine phosphorylation of paxillin, also restored paxillin levels, indicating a role for these kinases in the CD45-dependent regulation of paxillin. These data demonstrate that CD45 functions to regulate Pyk2/FAK activity, likely through the activity of Src family kinases, which in turn regulates the levels of paxillin to modulate macrophage adhesion and migration.
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