ArticlePloS one2013
Protein phosphatase 1ß limits ring canal constriction during Drosophila germline cyst formation.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Bridges under construction: the dynamics of ring canal expansion during Drosophila oogenesis.Developmental biology · 2026Review
- A tug-of-war between germ cell motility and intercellular bridges controls germline cyst formation in mice.Current biology : CB · 2024Article
- Lineage-based scaling of germline intercellular bridges during oogenesis.Development (Cambridge, England) · 2024Article
- From network analysis to experimental validation: identification of regulators of non-muscle myosin II contractility using the folded-gastrulation signaling pathway.BMC molecular and cell biology · 2023Article
- Mechanics of stabilized intercellular bridges.Biophysical journal · 2022Article
- Protein phosphatase 1 activity controls a balance between collective and single cell modes of migration.eLife · 2020Article
- The Arp2/3 complex and the formin, Diaphanous, are both required to regulate the size of germline ring canals in the developing egg chamber.Developmental biology · 2020Article
- A Genetic Screen for Genes That Impact Peroxisomes inG3 (Bethesda, Md.) · 2020Article
- Proximity labeling reveals novel interactomes in liveDevelopment (Cambridge, England) · 2019Article
- The Misshapen kinase regulates the size and stability of the germline ring canals in the Drosophila egg chamber.Developmental biology · 2018Article
- Suppression of epithelial folding at actomyosin-enriched compartment boundaries downstream of Wingless signalling inDevelopment (Cambridge, England) · 2018Article
- A biochemical network controlling basal myosin oscillation.Nature communications · 2018Article
- Unraveling Novel Mechanisms of Neurodegeneration Through a Large-Scale Forward Genetic Screen inFrontiers in genetics · 2018Review
- Recent advances in cerebral cavernous malformation research.Vessel plus · 2018Article
- Review
- A Sterile 20 Family Kinase and Its Co-factor CCM-3 Regulate Contractile Ring Proteins on Germline Intercellular Bridges.Current biology : CB · 2017Article
- Analysis of the cytoskeleton organization and its possible functions in male earthworm germ-line cysts equipped with a cytophore.Cell and tissue research · 2016Article
- Myosin light-chain phosphatase regulates basal actomyosin oscillations during morphogenesis.Nature communications · 2016Article
- Functional specialization of chordate CDK1 paralogs during oogenic meiosis.Cell cycle (Georgetown, Tex.) · 2015Article
- Analysis of the expression patterns, subcellular localisations and interaction partners of Drosophila proteins using a pigP protein trap library.Development (Cambridge, England) · 2014Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Germline cyst formation is essential for the propagation of many organisms including humans and flies. The cytoplasm of germline cyst cells communicate with each other directly via large intercellular bridges called ring canals. Ring canals are often derived from arrested contractile rings during incomplete cytokinesis. However how ring canal formation, maintenance and growth are regulated remains unclear. To better understand this process, we carried out an unbiased genetic screen in Drosophila melanogaster germ cells and identified multiple alleles of flapwing (flw), a conserved serine/threonine-specific protein phosphatase. Flw had previously been reported to be unnecessary for early D. melanogaster oogenesis using a hypomorphic allele. We found that loss of Flw leads to over-constricted nascent ring canals and subsequently tiny mature ring canals, through which cytoplasmic transfer from nurse cells to the oocyte is impaired, resulting in small, non-functional eggs. Flw is expressed in germ cells undergoing incomplete cytokinesis, completely colocalized with the Drosophila myosin binding subunit of myosin phosphatase (DMYPT). This colocalization, together with genetic interaction studies, suggests that Flw functions together with DMYPT to negatively regulate myosin activity during ring canal formation. The identification of two subunits of the tripartite myosin phosphatase as the first two main players required for ring canal constriction indicates that tight regulation of myosin activity is essential for germline cyst formation and reproduction in D. melanogaster and probably other species as well.
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