ArticlePloS one2013
Regulation of flagellar biogenesis by a calcium dependent protein kinase in Chlamydomonas reinhardtii.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 47 citations in OpenAlex.
- Article
- The flagellar length control system: exploring the physical biology of organelle size.Physical biology · 2023Review
- Article
- Article
- Structural Basis for the Functional Diversity of Centrins: A Focus on Calcium Sensing Properties and Target Recognition.International journal of molecular sciences · 2021Review
- Chlamydomonas reinhardtii cellular compartments and their contribution to intracellular calcium signalling.Journal of experimental botany · 2021Review
- IFT54 directly interacts with kinesin-II and IFT dynein to regulate anterograde intraflagellar transport.The EMBO journal · 2021Article
- HAP2-Mediated Gamete Fusion: Lessons From the World of Unicellular Eukaryotes.Frontiers in cell and developmental biology · 2021Review
- Functional exploration of heterotrimeric kinesin-II in IFT and ciliary length control ineLife · 2020Article
- FLS2 is a CDK-like kinase that directly binds IFT70 and is required for proper ciliary disassembly in Chlamydomonas.PLoS genetics · 2020Article
- Potassium channel KCN11 is required for maintaining cellular osmolarity during nitrogen starvation to control proper cell physiology and TAG accumulation inBiotechnology for biofuels · 2020Article
- Chlamydomonas WDR92 in association with R2TP-like complex and multiple DNAAFs to regulate ciliary dynein preassembly.Journal of molecular cell biology · 2019Article
- Channelrhodopsin-1 Phosphorylation Changes with Phototactic Behavior and Responds to Physiological Stimuli inThe Plant cell · 2019Article
- A global analysis of IFT-A function reveals specialization for transport of membrane-associated proteins into cilia.Journal of cell science · 2019Article
- Rapid and high efficiency transformation ofBioscience reports · 2019Article
- Nourseothricin N-acetyl transferase (NAT), a new selectable marker for nuclear gene expression inPlant methods · 2019Article
- IFT54 regulates IFT20 stability but is not essential for tubulin transport during ciliogenesis.Cellular and molecular life sciences : CMLS · 2017Article
- Functional exploration of the IFT-A complex in intraflagellar transport and ciliogenesis.PLoS genetics · 2017Article
- Mechanism of ciliary disassembly.Cellular and molecular life sciences : CMLS · 2016Review
- A NIMA-related kinase, CNK4, regulates ciliary stability and length.Molecular biology of the cell · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chlamydomonas reinhardtii, a bi-flagellated green alga, is a model organism for studies of flagella or cilia related activities including cilia-based signaling, flagellar motility and flagellar biogenesis. Calcium has been shown to be a key regulator of these cellular processes whereas the signaling pathways linking calcium to these cellular functions are less understood. Calcium-dependent protein kinases (CDPKs), which are present in plants but not in animals, are also present in ciliated microorganisms which led us to examine their possible functions and mechanisms in flagellar related activities. By in silico analysis of Chlamydomonas genome we have identified 14 CDPKs and studied one of the flagellar localized CDPKs--CrCDPK3. CrCDPK3 was a protein of 485 amino acids and predicted to have a protein kinase domain at the N-terminus and four EF-hand motifs at the C-terminus. In flagella, CrCDPK3 was exclusively localized in the membrane matrix fraction and formed an unknown 20 S protein complex. Knockdown of CrCDPK3 expression by using artificial microRNA did not affect flagellar motility as well as flagellar adhesion and mating. Though flagellar shortening induced by treatment with sucrose or sodium pyrophosphate was not affected in RNAi strains, CrCDPK3 increased in the flagella, and pre-formed protein complex was disrupted. During flagellar regeneration, CrCDPK3 also increased in the flagella. When extracellular calcium was lowered to certain range by the addition of EGTA after deflagellation, flagellar regeneration was severely affected in RNAi cells compared with wild type cells. In addition, during flagellar elongation induced by LiCl, RNAi cells exhibited early onset of bulbed flagella. This work expands new functions of CDPKs in flagellar activities by showing involvement of CrCDPK3 in flagellar biogenesis in Chlamydomonas.
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