ArticlePloS one2013
Characterization of cell subpopulations expressing progenitor cell markers in porcine cardiac valves.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 29 citations in OpenAlex.
- Tissue response and clinical outcomes after cardiovascular use of porcine small intestinal small intestinal submucosal extracellular matrix: a systematic review.Frontiers in cardiovascular medicine · 2025Pooled it
- Unveiling the intricacies of cardiac valve pathophysiology.Frontiers in cardiovascular medicine · 2025Review
- Quantifying Cell Heterogeneity and Subpopulations Using Single Cell Metabolomics.Analytical chemistry · 2023Article
- Human and porcine aortic valve endothelial and interstitial cell isolation and characterization.Frontiers in cardiovascular medicine · 2023Article
- High resolution monitoring of valvular interstitial cell driven pathomechanisms in procalcific environment using label-free impedance spectroscopy.Frontiers in cardiovascular medicine · 2023Article
- Profiling natural serum antibodies of non-human primates with a carbohydrate antigen microarray.Xenotransplantation · 2020Article
- Comorbid TNF-mediated heart valve disease and chronic polyarthritis share common mesenchymal cell-mediated aetiopathogenesis.Annals of the rheumatic diseases · 2018Article
- Valve Interstitial Cells: The Key to Understanding the Pathophysiology of Heart Valve Calcification.Journal of the American Heart Association · 2017Review
- Fibroblast-Specific Genetic Manipulation of p38 Mitogen-Activated Protein Kinase In Vivo Reveals Its Central Regulatory Role in Fibrosis.Circulation · 2017Article
- Optimizing Photo-Encapsulation Viability of Heart Valve Cell Types in 3D Printable Composite Hydrogels.Annals of biomedical engineering · 2017Article
- In vitro 3D model and miRNA drug delivery to target calcific aortic valve disease.Clinical science (London, England : 1979) · 2017Review
- Valve Interstitial Cells Act in a Pericyte Manner Promoting Angiogensis and Invasion by Valve Endothelial Cells.Annals of biomedical engineering · 2016Article
- Dynamic stiffening of poly(ethylene glycol)-based hydrogels to direct valvular interstitial cell phenotype in a three-dimensional environment.Biomaterials · 2015Article
- Regulation of valve endothelial cell vasculogenic network architectures with ROCK and Rac inhibitors.Microvascular research · 2015Article
- Cross Talk between NOTCH Signaling and Biomechanics in Human Aortic Valve Disease Pathogenesis.Journal of cardiovascular development and disease · 2014Article
- Cardiac valve cells and their microenvironment--insights from in vitro studies.Nature reviews. Cardiology · 2014Review
- Roles of transforming growth factor-β1 and OB-cadherin in porcine cardiac valve myofibroblast differentiation.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2014Article
- Advances in the treatment of aortic valve disease: is it time for companion diagnostics?Current opinion in pediatrics · 2014Review
- The role of valvular endothelial cell paracrine signaling and matrix elasticity on valvular interstitial cell activation.Biomaterials · 2014Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Valvular interstitial cells (VICs) are the main population of cells found in cardiac valves. These resident fibroblastic cells play important roles in maintaining proper valve function, and their dysregulation has been linked to disease progression in humans. Despite the critical functions of VICs, their cellular composition is still not well defined for humans and other mammals. Given the limited availability of healthy human valves and the similarity in valve structure and function between humans and pigs, we characterized porcine VICs (pVICs) based on expression of cell surface proteins and sorted a specific subpopulation of pVICs to study its functions. We found that small percentages of pVICs express the progenitor cell markers ABCG2 (~5%), NG2 (~5%) or SSEA-4 (~7%), whereas another subpopulation (~5%) expresses OB-CDH, a type of cadherin expressed by myofibroblasts or osteo-progenitors. pVICs isolated from either aortic or pulmonary valves express most of these protein markers at similar levels. Interestingly, OB-CDH, NG2 and SSEA-4 all label distinct valvular subpopulations relative to each other; however, NG2 and ABCG2 are co-expressed in the same cells. ABCG2(+) cells were further characterized and found to deposit more calcified matrix than ABCG2(-) cells upon osteogenic induction, suggesting that they may be involved in the development of osteogenic VICs during valve pathology. Cell profiling based on flow cytometry and functional studies with sorted primary cells provide not only new and quantitative information about the cellular composition of porcine cardiac valves, but also contribute to our understanding of how a subpopulation of valvular cells (ABCG2(+) cells) may participate in tissue repair and disease progression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.