ArticlePloS one2013
Quantitative analysis of cell migration using optical flow.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
23 citing papers in PubMed, 36 citations in OpenAlex.
- Review
- Exosome-shuttled miR-126 mediates ethanol-induced disruption of neural crest cell-placode cell interaction by targeting SDF1.Toxicological sciences : an official journal of the Society of Toxicology · 2023Article
- Neural crest cells and fetal alcohol spectrum disorders: Mechanisms and potential targets for prevention.Pharmacological research · 2023Review
- Subpopulations of hypocretin/orexin neurons differ in measures of their cell proliferation, dynorphin co-expression, projections, and response to embryonic ethanol exposure.Scientific reports · 2023Article
- Article
- Embryonic ethanol exposure induces ectopic Hcrt and MCH neurons outside hypothalamus in rats and zebrafish: Role in ethanol-induced behavioural disturbances.Addiction biology · 2022Article
- Up-regulation of microRNA-34a mediates ethanol-induced impairment of neural crest cell migration in vitro and in zebrafish embryos through modulating epithelial-mesenchymal transition by targeting Snail1.Toxicology letters · 2022Article
- Zebrafish models of fetal alcohol spectrum disorders.Genesis (New York, N.Y. : 2000) · 2021Review
- Neural crest metabolism: At the crossroads of development and disease.Developmental biology · 2021Review
- An integrated platform to facilitate the calculation, validation and visualization of optical flow velocities in biological images.Journal of the Royal Society, Interface · 2021Article
- Optical flow analysis reveals that Kinesin-mediated advection impacts the orientation of microtubules in theMolecular biology of the cell · 2020Article
- Maternal ethanol consumption before paternal fertilization: Stimulation of hypocretin neurogenesis and ethanol intake in zebrafish offspring.Progress in neuro-psychopharmacology & biological psychiatry · 2020Article
- Embryonic Ethanol Exposure Affects the Early Development, Migration, and Location of Hypocretin/Orexin Neurons in Zebrafish.Alcoholism, clinical and experimental research · 2019Article
- Alcohol-mediated calcium signals dysregulate pro-survival Snai2/PUMA/Bcl2 networks to promote p53-mediated apoptosis in avian neural crest progenitors.Birth defects research · 2019Article
- Dancing Styles of Collective Cell Migration: Image-Based Computational Analysis of JRAB/MICAL-L2.Frontiers in cell and developmental biology · 2018Review
- Extrinsic mechanical forces mediate retrograde axon extension in a developing neuronal circuit.Nature communications · 2017Article
- Embryonic aortic arch hemodynamics are a functional biomarker for ethanol-induced congenital heart defects [Invited].Biomedical optics express · 2017Article
- Quantitative Analysis of Intracellular Motility Based on Optical Flow Model.Journal of healthcare engineering · 2017Article
- Olfactory sensory system develops from coordinated movements within the neural plate.Developmental dynamics : an official publication of the American Association of Anatomists · 2014Article
- 5-Mehtyltetrahydrofolate rescues alcohol-induced neural crest cell migration abnormalities.Molecular brain · 2014Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Neural crest cells exhibit dramatic migration behaviors as they populate their distant targets. Using a line of zebrafish expressing green fluorescent protein (sox10:EGFP) in neural crest cells we developed an assay to analyze and quantify cell migration as a population, and use it here to characterize in detail the subtle defects in cell migration caused by ethanol exposure during early development. The challenge was to quantify changes in the in vivo migration of all Sox10:EGFP expressing cells in the visual field of time-lapse movies. To perform this analysis we used an Optical Flow algorithm for motion detection and combined the analysis with a fit to an affine transformation. Through this analysis we detected and quantified significant differences in the cell migrations of Sox10:EGFP positive cranial neural crest populations in ethanol treated versus untreated embryos. Specifically, treatment affected migration by increasing the left-right asymmetry of the migrating cells and by altering the direction of cell movements. Thus, by applying this novel computational analysis, we were able to quantify the movements of populations of cells, allowing us to detect subtle changes in cell behaviors. Because cranial neural crest cells contribute to the formation of the frontal mass these subtle differences may underlie commonly observed facial asymmetries in normal human populations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.