ArticlePloS one2013
Molecular characterization of multidrug resistant hospital isolates using the antimicrobial resistance determinant microarray.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 28 citations in OpenAlex.
- A comparison of various feature extraction and machine learning methods for antimicrobial resistance prediction inFrontiers in antibiotics · 2023Article
- eMIC-AntiKP: Estimating minimum inhibitory concentrations of antibiotics towardsComputational and structural biotechnology journal · 2023Article
- Immunization of Rabbits with a QuadrivalentmSphere · 2022Article
- Tracking Antimicrobial Resistance Determinants in Diarrheal Pathogens: A Cross-Institutional Pilot Study.International journal of molecular sciences · 2020Article
- A Survey of Antimicrobial Resistance Determinants in Category A Select Agents, Exempt Strains, and Near-Neighbor Species.International journal of molecular sciences · 2020Article
- Nosocomial transmission of extensively drug resistant Acinetobacter baumannii strains in a tertiary level hospital.PloS one · 2020Article
- CLSI based antibiogram profile and the detection of MDR and XDR strains ofMedical journal of the Islamic Republic of Iran · 2019Article
- Detection of antimicrobial resistance genes associated with the International Space Station environmental surfaces.Scientific reports · 2018Article
- Developing an in silico minimum inhibitory concentration panel test for Klebsiella pneumoniae.Scientific reports · 2018Article
- Antimicrobial resistance of Klebsiella pneumoniae stool isolates circulating in Kenya.PloS one · 2017Article
- Antimicrobial Resistance Prediction in PATRIC and RAST.Scientific reports · 2016Article
- Uropathogenic Escherichia coli virulence genes: invaluable approaches for designing DNA microarray probes.Central European journal of urology · 2015Review
- Antimicrobial resistance determinants in Acinetobacter baumannii isolates taken from military treatment facilities.Antimicrobial agents and chemotherapy · 2014Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Molecular methods that enable the detection of antimicrobial resistance determinants are critical surveillance tools that are necessary to aid in curbing the spread of antibiotic resistance. In this study, we describe the use of the Antimicrobial Resistance Determinant Microarray (ARDM) that targets 239 unique genes that confer resistance to 12 classes of antimicrobial compounds, quaternary amines and streptothricin for the determination of multidrug resistance (MDR) gene profiles. Fourteen reference MDR strains, which either were genome, sequenced or possessed well characterized drug resistance profiles were used to optimize detection algorithms and threshold criteria to ensure the microarray's effectiveness for unbiased characterization of antimicrobial resistance determinants in MDR strains. The subsequent testing of Acinetobacter baumannii, Escherichia coli and Klebsiella pneumoniae hospital isolates revealed the presence of several antibiotic resistance genes [e.g. belonging to TEM, SHV, OXA and CTX-M classes (and OXA and CTX-M subfamilies) of β-lactamases] and their assemblages which were confirmed by PCR and DNA sequence analysis. When combined with results from the reference strains, ~25% of the ARDM content was confirmed as effective for representing allelic content from both Gram-positive and -negative species. Taken together, the ARDM identified MDR assemblages containing six to 18 unique resistance genes in each strain tested, demonstrating its utility as a powerful tool for molecular epidemiological investigations of antimicrobial resistance in clinically relevant bacterial pathogens.
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