ArticlePLoS genetics2013
Genome wide association identifies novel loci involved in fungal communication.
Article in PLoS genetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed.
- Identification of regions required for allelic specificity at the cell wall remodeling allorecognition checkpoint in Neurospora crassa.Genetics · 2025Article
- Decoding fungal communication networks: molecular signaling, genetic regulation, and ecological implications.Functional & integrative genomics · 2025Review
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- Looking outside the box: a comparative cross-kingdom view on the cell biology of the three major lineages of eukaryotic multicellular life.Cellular and molecular life sciences : CMLS · 2023Review
- Spermidine Synthase and Saccharopine Reductase Have Co-Expression Patterns Both in Basidiomycetes with Fusion Form and Ascomycetes with Separate Form.Journal of fungi (Basel, Switzerland) · 2023Article
- Heritability and gene functions associated with sclerotia formation ofMicrobial genomics · 2023Article
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- Genomic and Molecular Identification of Genes Contributing to the Caspofungin Paradoxical Effect inMicrobiology spectrum · 2022Article
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- Coccidioides Species: A Review of Basic Research: 2022.Journal of fungi (Basel, Switzerland) · 2022Review
- Population genomics provides insights into the genetic basis of adaptive evolution in the mushroom-forming fungusJournal of advanced research · 2022Article
- CaJournal of fungi (Basel, Switzerland) · 2022Article
- Differential Physiological Prerequisites and Gene Expression Profiles of Conidial Anastomosis Tube and Germ Tube Formation inJournal of fungi (Basel, Switzerland) · 2021Article
- Article
- Wild Isolates ofMicroorganisms · 2020Article
- Establishment of Neurospora crassa as a model organism for fungal virology.Nature communications · 2020Article
- Musashi expression in intestinal stem cells attenuates radiation-induced decline in intestinal permeability and survival in Drosophila.Scientific reports · 2020Article
- Occurrence and possible roles of polysaccharides in fungi and their influence on the development of new technologies.Carbohydrate polymers · 2020Review
- Natural variation of root lesion nematode antagonism in the biocontrol fungusEvolutionary applications · 2020Article
- Neurospora from Natural Populations: Population Genomics Insights into the Life History of a Model Microbial Eukaryote.Methods in molecular biology (Clifton, N.J.) · 2020Review
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7 authors.
Funding
Abstract
Understanding how genomes encode complex cellular and organismal behaviors has become the outstanding challenge of modern genetics. Unlike classical screening methods, analysis of genetic variation that occurs naturally in wild populations can enable rapid, genome-scale mapping of genotype to phenotype with a medium-throughput experimental design. Here we describe the results of the first genome-wide association study (GWAS) used to identify novel loci underlying trait variation in a microbial eukaryote, harnessing wild isolates of the filamentous fungus Neurospora crassa. We genotyped each of a population of wild Louisiana strains at 1 million genetic loci genome-wide, and we used these genotypes to map genetic determinants of microbial communication. In N. crassa, germinated asexual spores (germlings) sense the presence of other germlings, grow toward them in a coordinated fashion, and fuse. We evaluated germlings of each strain for their ability to chemically sense, chemotropically seek, and undergo cell fusion, and we subjected these trait measurements to GWAS. This analysis identified one gene, NCU04379 (cse-1, encoding a homolog of a neuronal calcium sensor), at which inheritance was strongly associated with the efficiency of germling communication. Deletion of cse-1 significantly impaired germling communication and fusion, and two genes encoding predicted interaction partners of CSE1 were also required for the communication trait. Additionally, mining our association results for signaling and secretion genes with a potential role in germling communication, we validated six more previously unknown molecular players, including a secreted protease and two other genes whose deletion conferred a novel phenotype of increased communication and multi-germling fusion. Our results establish protein secretion as a linchpin of germling communication in N. crassa and shed light on the regulation of communication molecules in this fungus. Our study demonstrates the power of population-genetic analyses for the rapid identification of genes contributing to complex traits in microbial species.
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