ArticlePLoS pathogens2013
A novel role for pro-coagulant microvesicles in the early host defense against streptococcus pyogenes.
Article in PLoS pathogens, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 44 citations in OpenAlex.
- A Fraction ofInternational journal of molecular sciences · 2025Article
- Selective but not pan-CDK inhibition abrogates 5-FU-driven tissue factor upregulation in colon cancer.Scientific reports · 2024Article
- The Recruitment and Activation of Plasminogen by Bacteria-The Involvement in Chronic Infection Development.International journal of molecular sciences · 2023Review
- Extracellular Vesicles: A Double-Edged Sword in Sepsis.Pharmaceuticals (Basel, Switzerland) · 2021Review
- Luminal microvesicles uniquely influence translocating bacteria after SIV infection.Mucosal immunology · 2021Article
- Neutrophils produce proinflammatory or anti-inflammatory extracellular vesicles depending on the environmental conditions.Journal of leukocyte biology · 2021Article
- Membrane Microvesicles as Potential Vaccine Candidates.International journal of molecular sciences · 2021Review
- Non-coding RNAs and Exosomes: Their Role in the Pathogenesis of Sepsis.Molecular therapy. Nucleic acids · 2020Review
- Kininogen supports inflammation and bacterial spreading during Streptococccus Pyogenes Sepsis.EBioMedicine · 2020Article
- Exosome: A New Player in Translational Nanomedicine.Journal of clinical medicine · 2020Review
- The contact system proteases play disparate roles in streptococcal sepsis.Haematologica · 2020Article
- Role of Mac-1 integrin in generation of extracellular vesicles with antibacterial capacity from neutrophilic granulocytes.Journal of extracellular vesicles · 2020Article
- Inflammatory Joint Disease Is a Risk Factor for Streptococcal Sepsis and Septic Arthritis in Mice.Frontiers in immunology · 2020Article
- Different Calcium and Src Family Kinase Signaling in Mac-1 Dependent Phagocytosis and Extracellular Vesicle Generation.Frontiers in immunology · 2019Article
- Exosomes and microvesicles in normal physiology, pathophysiology, and renal diseases.Pediatric nephrology (Berlin, Germany) · 2019Review
- Role of extracellular vesicles in the development of sepsis-induced coagulopathy.Journal of intensive care · 2018Review
- Extracellular Vesicles as Markers and Mediators in Sepsis.Theranostics · 2018Review
- Interaction of the Human Contact System with Pathogens-An Update.Frontiers in immunology · 2018Review
- Review
- Biology and biogenesis of shed microvesicles.Small GTPases · 2017Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previous studies have shown that stimulation of whole blood or peripheral blood mononuclear cells with bacterial virulence factors results in the sequestration of pro-coagulant microvesicles (MVs). These particles explore their clotting activity via the extrinsic and intrinsic pathway of coagulation; however, their pathophysiological role in infectious diseases remains enigmatic. Here we describe that the interaction of pro-coagulant MVs with bacteria of the species Streptococcus pyogenes is part of the early immune response to the invading pathogen. As shown by negative staining electron microscopy and clotting assays, pro-coagulant MVs bind in the presence of plasma to the bacterial surface. Fibrinogen was identified as a linker that, through binding to the M1 protein of S. pyogenes, allows the opsonization of the bacteria by MVs. Surface plasmon resonance analysis revealed a strong interaction between pro-coagulant MVs and fibrinogen with a KD value in the nanomolar range. When performing a mass-spectrometry-based strategy to determine the protein quantity, a significant up-regulation of the fibrinogen-binding integrins CD18 and CD11b on pro-coagulant MVs was recorded. Finally we show that plasma clots induced by pro-coagulant MVs are able to prevent bacterial dissemination and possess antimicrobial activity. These findings were confirmed by in vivo experiments, as local treatment with pro-coagulant MVs dampens bacterial spreading to other organs and improved survival in an invasive streptococcal mouse model of infection. Taken together, our data implicate that pro-coagulant MVs play an important role in the early response of the innate immune system in infectious diseases.
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