ArticleNucleic acids research2013
Universal digital high-resolution melt: a novel approach to broad-based profiling of heterogeneous biological samples.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 43 citations in OpenAlex.
- Cross-kingdom pathogen detection via duplex universal PCR and high-resolution melt.Biosensors & bioelectronics · 2025Article
- Universal digital high-resolution melting for the detection of pulmonary mold infections.Journal of clinical microbiology · 2024Article
- Improving bacteria identification from digital melt assay via oligonucleotide-based temperature calibration.Analytica chimica acta · 2024Article
- Next-generation molecular diagnostics: Leveraging digital technologies to enhance multiplexing in real-time PCR.Trends in analytical chemistry : TRAC · 2023Article
- Next generation multiplexing for digital PCR using a novel melt-based hairpin probe design.Frontiers in genetics · 2023Article
- Diagnosis of Bloodstream Infections: An Evolution of Technologies towards Accurate and Rapid Identification and Antibiotic Susceptibility Testing.Antibiotics (Basel, Switzerland) · 2022Review
- Improving Quantitative Power in Digital PCR through Digital High-Resolution Melting.Journal of clinical microbiology · 2020Article
- Direct-from-Specimen Pathogen Identification: Evolution of Syndromic Panels.Clinics in laboratory medicine · 2019Review
- Emerging Analytical Techniques for Rapid Pathogen Identification and Susceptibility Testing.Annual review of analytical chemistry (Palo Alto, Calif.) · 2019Review
- A "Culture" Shift: Broad Bacterial Detection, Identification, and Antimicrobial Susceptibility Testing Directly from Whole Blood.Clinical chemistry · 2018Article
- Emerging Technologies for Molecular Diagnosis of Sepsis.Clinical microbiology reviews · 2018Review
- Massively parallel digital high resolution melt for rapid and absolutely quantitative sequence profiling.Scientific reports · 2017Article
- Microbial Typing by Machine Learned DNA Melt Signatures.Scientific reports · 2017Article
- Nested Machine Learning Facilitates Increased Sequence Content for Large-Scale Automated High Resolution Melt Genotyping.Scientific reports · 2016Article
- Multi-template polymerase chain reaction.Biomolecular detection and quantification · 2014Review
- Melting analysis on microbeads in rapid temperature-gradient inside microchannels for single nucleotide polymorphisms detection.Biomicrofluidics · 2014Article
- Trainable high resolution melt curve machine learning classifier for large-scale reliable genotyping of sequence variants.PloS one · 2014Article
- A 'culture' shift: Application of molecular techniques for diagnosing polymicrobial infections.Biotechnology advancesReview
Corrections and comments
- Erratum issuedUniversal digital high-resolution melt: a novel approach to broad-based profiling of heterogeneous biological samples.2016 · Jo Masek, Billie [Corrected to Masek, Billie J]
Authors and funding
11 authors at 2 institutions in 1 country.
Funding
Abstract
Comprehensive profiling of nucleic acids in genetically heterogeneous samples is important for clinical and basic research applications. Universal digital high-resolution melt (U-dHRM) is a new approach to broad-based PCR diagnostics and profiling technologies that can overcome issues of poor sensitivity due to contaminating nucleic acids and poor specificity due to primer or probe hybridization inaccuracies for single nucleotide variations. The U-dHRM approach uses broad-based primers or ligated adapter sequences to universally amplify all nucleic acid molecules in a heterogeneous sample, which have been partitioned, as in digital PCR. Extensive assay optimization enables direct sequence identification by algorithm-based matching of melt curve shape and Tm to a database of known sequence-specific melt curves. We show that single-molecule detection and single nucleotide sensitivity is possible. The feasibility and utility of U-dHRM is demonstrated through detection of bacteria associated with polymicrobial blood infection and microRNAs (miRNAs) associated with host response to infection. U-dHRM using broad-based 16S rRNA gene primers demonstrates universal single cell detection of bacterial pathogens, even in the presence of larger amounts of contaminating bacteria; U-dHRM using universally adapted Lethal-7 miRNAs in a heterogeneous mixture showcases the single copy sensitivity and single nucleotide specificity of this approach.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.