ArticleNucleic acids research2013
Genome-wide single-cell-level screen for protein abundance and localization changes in response to DNA damage in S. cerevisiae.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 45 citations in OpenAlex.
- Article
- Quantitative Characterisation of Low Abundant Yeast Mitochondrial Proteins Reveals Compensation for Haplo-Insufficiency in Different Environments.International journal of molecular sciences · 2022Article
- Single-cell image analysis to explore cell-to-cell heterogeneity in isogenic populations.Cell systems · 2021Review
- Proteomic analysis of the S. cerevisiae response to the anticancer ruthenium complex KP1019.Metallomics : integrated biometal science · 2020Article
- Incorporation of a unified protein abundance dataset into the Saccharomyces genome database.Database : the journal of biological databases and curation · 2020Article
- A quantitative screen for metabolic enzyme structures reveals patterns of assembly across the yeast metabolic network.Molecular biology of the cell · 2019Article
- Transcriptional responses to DNA damage.DNA repair · 2019Review
- Spatial proteomics: a powerful discovery tool for cell biology.Nature reviews. Molecular cell biology · 2019Review
- Definition of the Minimal Contents for the Molecular Simulation of the Yeast Cytoplasm.Frontiers in molecular biosciences · 2019Article
- Doxorubicin-induced DNA Damage Causes Extensive Ubiquitination of Ribosomal Proteins Associated with a Decrease in Protein Translation.Molecular & cellular proteomics : MCP · 2018Article
- Database for High Throughput Screening Hits (dHITS): a simple tool to retrieve gene specific phenotypes from systematic screens done in yeast.Yeast (Chichester, England) · 2018Article
- Measuring cell cycle-dependent DNA damage responses and p53 regulation on a cell-by-cell basis from image analysis.Cell cycle (Georgetown, Tex.) · 2018Article
- Selective aggregation of the splicing factor Hsh155 suppresses splicing upon genotoxic stress.The Journal of cell biology · 2017Article
- P-body proteins regulate transcriptional rewiring to promote DNA replication stress resistance.Nature communications · 2017Article
- Early-onset torsion dystonia: a novel high-throughput yeast genetic screen for factors modifying protein levels of torsinAΔE.Disease models & mechanisms · 2017Article
- Genome-wide and protein kinase-focused RNAi screens reveal conserved and novel damage response pathways in Trypanosoma brucei.PLoS pathogens · 2017Article
- A Critical and Comparative Review of Fluorescent Tools for Live-Cell Imaging.Annual review of physiology · 2017Review
- MTE1 Functions with MPH1 in Double-Strand Break Repair.Genetics · 2016Article
- Time series modeling of live-cell shape dynamics for image-based phenotypic profiling.Integrative biology : quantitative biosciences from nano to macro · 2016Article
- Ndd1 turnover by SCF(Grr1) is inhibited by the DNA damage checkpoint in Saccharomyces cerevisiae.PLoS genetics · 2015Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
An effective response to DNA damaging agents involves modulating numerous facets of cellular homeostasis in addition to DNA repair and cell-cycle checkpoint pathways. Fluorescence microscopy-based imaging offers the opportunity to simultaneously interrogate changes in both protein level and subcellular localization in response to DNA damaging agents at the single-cell level. We report here results from screening the yeast Green Fluorescent Protein (GFP)-fusion library to investigate global cellular protein reorganization on exposure to the alkylating agent methyl methanesulfonate (MMS). Broad groups of induced, repressed, nucleus- and cytoplasm-enriched proteins were identified. Gene Ontology and interactome analyses revealed the underlying cellular processes. Transcription factor (TF) analysis identified principal regulators of the response, and targets of all major stress-responsive TFs were enriched amongst the induced proteins. An unexpected partitioning of biological function according to the number of TFs targeting individual genes was revealed. Finally, differential modulation of ribosomal proteins depending on methyl methanesulfonate dose was shown to correlate with cell growth and with the translocation of the Sfp1 TF. We conclude that cellular responses can navigate different routes according to the extent of damage, relying on both expression and localization changes of specific proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.