ArticleNucleic acids research2013
Tagetitoxin inhibits transcription by stabilizing pre-translocated state of the elongation complex.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 30 citations in OpenAlex.
- Genomic and mutational analysis of Pseudomonas syringae pv. tagetis EB037 pathogenicity on sunflower.BMC microbiology · 2025Article
- Single-molecule studies reveal the off-pathway early paused state intermediates as a target of streptolydigin inhibition of RNA polymerase and its dramatic enhancement by Gre factors.Nucleic acids research · 2025Article
- Structure and function of the Si3 insertion integrated into the trigger loop/helix of cyanobacterial RNA polymerase.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Two distinct pathways of RNA polymerase backtracking determine the requirement for the Trigger Loop during RNA hydrolysis.Nucleic acids research · 2021Article
- Ideality in Context: Motivations for Total Synthesis.Accounts of chemical research · 2021Article
- Ureidothiophene inhibits interaction of bacterial RNA polymerase with -10 promotor element.Nucleic acids research · 2020Article
- Total Synthesis of Tagetitoxin.Journal of the American Chemical Society · 2020Article
- Evolving MRSA: High-level β-lactam resistance in Staphylococcus aureus is associated with RNA Polymerase alterations and fine tuning of gene expression.PLoS pathogens · 2020Article
- Structural Basis of Transcription: RNA Polymerase Backtracking and Its Reactivation.Molecular cell · 2019Article
- RNA-DNA and DNA-DNA base-pairing at the upstream edge of the transcription bubble regulate translocation of RNA polymerase and transcription rate.Nucleic acids research · 2018Article
- A link between transcription fidelity and pausing in vivo.Transcription · 2017Article
- Bacterial RNA Polymerase-DNA Interaction-The Driving Force of Gene Expression and the Target for Drug Action.Frontiers in molecular biosciences · 2016Review
- Bacterial global regulators DksA/ppGpp increase fidelity of transcription.Nucleic acids research · 2015Article
- Transcription. Response to Comment on "Mechanism of eukaryotic RNA polymerase III transcription termination".Science (New York, N.Y.) · 2014Article
- Coliphage HK022 Nun protein inhibits RNA polymerase translocation.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Interplay between the trigger loop and the F loop during RNA polymerase catalysis.Nucleic acids research · 2014Article
- Transcription elongation. Heterogeneous tracking of RNA polymerase and its biological implications.Transcription · 2014Review
- Antibiotic streptolydigin requires noncatalytic Mg2+ for binding to RNA polymerase.Antimicrobial agents and chemotherapy · 2014Article
- Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Transcription elongation consists of repetition of the nucleotide addition cycle: phosphodiester bond formation, translocation and binding of the next nucleotide. Inhibitor of multi-subunit RNA polymerase tagetitoxin (TGT) enigmatically slows down addition of nucleotides in a sequence-dependent manner, only at certain positions of the template. Here, we show that TGT neither affects chemistry of RNA synthesis nor induces backward translocation, nor competes with the nucleoside triphosphate (NTP) in the active center. Instead, TGT increases the stability of the pre-translocated state of elongation complex, thus slowing down addition of the following nucleotide. We show that the extent of inhibition directly depends on the intrinsic stability of the pre-translocated state. The dependence of translocation equilibrium on the transcribed sequence results in a wide distribution (~1-10(3)-fold) of inhibitory effects of TGT at different positions of the template, thus explaining sequence-specificity of TGT action. We provide biochemical evidence that, in pre-translocated state, TGT stabilizes folded conformation of the Trigger Loop, which inhibits forward and backward translocation of the complex. The results suggest that Trigger Loop folding in the pre-translocated state may serve to reduce back-tracking of the elongation complex. Overall, we propose that translocation may be a limiting and highly regulated step of RNA synthesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.