ArticleNucleic acids research2013
Robust gene silencing mediated by antisense small RNAs in the pathogenic protist Entamoeba histolytica.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 64 citations in OpenAlex.
- Exploring virulence and stress response inMicrobiology spectrum · 2025Article
- Work with me here: variations in genome content and emerging genetic tools in Entamoeba histolytica.Trends in parasitology · 2025Review
- Digestive exophagy of biofilms by intestinal amoeba and its impact on stress tolerance and cytotoxicity.NPJ biofilms and microbiomes · 2023Article
- Glycogen Metabolism and Its Role in Growth and Encystation inLife (Basel, Switzerland) · 2023Article
- Queuine Salvaging in the Human ParasiteCells · 2022Article
- The Role of Acetate Kinase in the Human ParasiteParasitologia (Basel, Switzerland) · 2022Article
- A Strategy for Gene Knockdown in Dinoflagellates.Microorganisms · 2022Article
- PI Kinase-EhGEF2-EhRho5 axis contributes to LPA stimulated macropinocytosis in Entamoeba histolytica.PLoS pathogens · 2022Article
- Ponatinib, Lestaurtinib, and mTOR/PI3K Inhibitors Are Promising Repurposing Candidates against Entamoeba histolytica.Antimicrobial agents and chemotherapy · 2022Article
- Are Metabolites From the Gut Microbiota Capable of Regulating Epigenetic Mechanisms in the Human ParasiteFrontiers in cell and developmental biology · 2022Review
- Growth and Genetic Manipulation of Entamoeba histolytica.Current protocols · 2022Article
- Establishment of quantitative RNAi-based forward genetics in Entamoeba histolytica and identification of genes required for growth.PLoS pathogens · 2021Article
- Two StAR-related lipid transfer proteins play specific roles in endocytosis, exocytosis, and motility in the parasitic protist Entamoeba histolytica.PLoS pathogens · 2021Article
- Article
- Development of a CRISPR/Cas9 system in Entamoeba histolytica: proof of concept.International journal for parasitology · 2021Article
- ArfX2 GTPase Regulates Trafficking From the Trans-Golgi to Lysosomes and Is Necessary for Liver Abscess Formation in the Protozoan ParasiteFrontiers in cellular and infection microbiology · 2021Article
- Identification of oligo-adenylated small RNAs in the parasite Entamoeba and a potential role for small RNA control.BMC genomics · 2020Article
- Characterization of Extracellular Vesicles from Entamoeba histolytica Identifies Roles in Intercellular Communication That Regulates Parasite Growth and Development.Infection and immunity · 2020Article
- Direct and high-throughput assays for human cell killing through trogocytosis by Entamoeba histolytica.Molecular and biochemical parasitology · 2020Article
- EhC2B, a C2 domain-containing protein, promotes erythrophagocytosis in Entamoeba histolytica via actin nucleation.PLoS pathogens · 2020Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
RNA interference uses small RNAs (sRNA), which target genes for sequence-specific silencing. The parasite Entamoeba histolytica contains an abundant repertoire of 27 nt antisense (AS) sRNA with 5'-polyphosphate termini, but their roles in regulating gene expression have not been well established. We demonstrate that a gene-coding region to which large numbers of AS sRNAs map can serve as a 'trigger' and silence the gene fused to it. Silencing is mediated by generation of AS sRNAs with 5'-polyphosphate termini that have sequence specificity to the fused gene. The mechanism of silencing is independent of the placement of the trigger relative to the silenced gene but is dependent on the sRNA concentration to the trigger. Silencing requires transcription of the trigger-gene fusion and is maintained despite loss of the trigger plasmid. We used this approach to silence multiple amebic genes, including an E. histolytica Myb gene, which is upregulated during oxidative stress response. Silencing of the EhMyb gene decreased parasite viability under oxidative stress conditions. Thus, we have developed a new tool for genetic manipulation in E. histolytica with many advantages over currently available technologies. Additionally, these data shed mechanistic insights into a eukaryotic RNA interference pathway with many novel aspects.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.