ArticleJournal of bacteriology2013
Deciphering the interplay between two independent functions of the small RNA regulator SgrS in Salmonella.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 44 citations in OpenAlex.
- Review
- Large Roles of Small Proteins.Annual review of microbiology · 2024Review
- Small RNAs Activate Salmonella Pathogenicity Island 1 by Modulating mRNA Stability through theJournal of bacteriology · 2023Article
- Glutamine synthetase mRNA releases sRNA from its 3'UTR to regulate carbon/nitrogen metabolic balance ineLife · 2022Article
- Synthetic dual-function RNA reveals features necessary for target regulation.Journal of bacteriology · 2021Article
- Translation inhibition from a distance: The small RNA SgrS silences a ribosomal protein S1-dependent enhancer.Molecular microbiology · 2020Article
- Stochastic Analysis Demonstrates the Dual Role of Hfq in ChaperoningFrontiers in molecular biosciences · 2020Article
- Cryptic-Prophage-Encoded Small Protein DicB ProtectsJournal of bacteriology · 2019Article
- Determinants of target prioritization and regulatory hierarchy for the bacterial small RNA SgrS.Molecular microbiology · 2019Article
- The small RNA RssR regulates myo-inositol degradation by Salmonella enterica.Scientific reports · 2018Article
- Dual-Function RNAs.Microbiology spectrum · 2018Review
- Translational regulation by bacterial small RNAs via an unusual Hfq-dependent mechanism.Nucleic acids research · 2018Article
- The RNA Complement of Outer Membrane Vesicles FromFrontiers in microbiology · 2018Article
- The Small Protein SgrT Controls Transport Activity of the Glucose-Specific Phosphotransferase System.Journal of bacteriology · 2017Article
- Nucleoid and cytoplasmic localization of small RNAs in Escherichia coli.Nucleic acids research · 2017Article
- Dual-function sRNA encoded peptide SR1P modulates moonlighting activity of B. subtilis GapA.RNA biology · 2016Article
- Horizontal transfer of DNA methylation patterns into bacterial chromosomes.Nucleic acids research · 2016Article
- Towards the complete small RNome ofMicrobial genomics · 2016Article
- Target activation by regulatory RNAs in bacteria.FEMS microbiology reviews · 2015Review
- The small RNA SgrS: roles in metabolism and pathogenesis of enteric bacteria.Frontiers in cellular and infection microbiology · 2014Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Bacterial dual-function small RNAs regulate gene expression by RNA-RNA base pairing and also code for small proteins. SgrS is a dual-function small RNA in Escherichia coli and Salmonella that is expressed under stress conditions associated with accumulation of sugar-phosphates, and its activity is crucial for growth during stress. The base-pairing function of SgrS regulates a number of mRNA targets, resulting in reduced uptake and enhanced efflux of sugars. SgrS also encodes the SgrT protein, which reduces sugar uptake by a mechanism that is independent of base pairing. While SgrS base-pairing activity has been characterized in detail, little is known about how base pairing and translation of sgrT are coordinated. In the current study, we utilized a series of mutants to determine how translation of sgrT affected the efficiency of base pairing-dependent regulation and vice versa. Mutations that abrogated sgrT translation had minimal effects on base-pairing activity. Conversely, mutations that impaired base-pairing interactions resulted in increased SgrT production. Furthermore, while ectopic overexpression of sgrS mutant alleles lacking only one of the two functions rescued cell growth under stress conditions, the SgrS base-pairing function alone was indispensable for growth rescue when alleles were expressed from the native locus. Collectively, the results suggest that during stress, repression of sugar transporter synthesis via base pairing with sugar transporter mRNAs is the first priority of SgrS. Subsequently, SgrT is made and acts on preexisting transporters. The combined action of these two functions produces an effective stress response.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.