ArticleJournal of bacteriology2013
Identification and characterization of RibN, a novel family of riboflavin transporters from Rhizobium leguminosarum and other proteobacteria.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 40 citations in OpenAlex.
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- Cu Transport by the Extended Family of CcoA-like Transporters (CalT) in Proteobacteria.Scientific reports · 2019Article
- Rare variants of the FMN riboswitch class inRNA (New York, N.Y.) · 2019Article
- A Riboflavin Transporter in Bdellovibrio exovorous JSS.Journal of molecular microbiology and biotechnology · 2019Article
- Structure-Activity Relationship of Flavin Analogues That Target the Flavin Mononucleotide Riboswitch.ACS chemical biology · 2018Article
- Transcriptomics reveals a cross-modulatory effect between riboflavin and iron and outlines responses to riboflavin biosynthesis and uptake in Vibrio cholerae.Scientific reports · 2018Article
- Overview on the Bacterial Iron-Riboflavin Metabolic Axis.Frontiers in microbiology · 2018Review
- A novel bifunctional transcriptional regulator of riboflavin metabolism in Archaea.Nucleic acids research · 2017Article
- The superfamily keeps growing: Identification in trypanosomatids of RibJ, the first riboflavin transporter family in protists.PLoS neglected tropical diseases · 2017Article
- Contribution of riboflavin supply pathways toGut pathogens · 2017Article
- Differential regulation of riboflavin supply genes inGut pathogens · 2017Article
- Functional characterization of a csoR-cueA divergon in Bradyrhizobium liaoningense CCNWSX0360, involved in copper, zinc and cadmium cotolerance.Scientific reports · 2016Article
- Metabolic response of Clostridium ljungdahlii to oxygen exposure.Applied and environmental microbiology · 2015Article
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rhizobia are symbiotic bacteria able to invade and colonize the roots of legume plants, inducing the formation of nodules, where bacteria reduce atmospheric nitrogen (N2) to ammonia (NH3). Riboflavin availability influences the capacity of rhizobia to survive in the rhizosphere and to colonize roots. In this study, we identified the RL1692 gene of Rhizobium leguminosarum downstream of a flavin mononucleotide (FMN) riboswitch. RL1692 encodes a putative transmembrane permease with two EamA domains. The presence of an FMN riboswitch regulating a transmembrane protein is usually observed in riboflavin transporters, suggesting that RL1692 may be involved in riboflavin uptake. The product of RL1692, which we named RibN, is conserved in members of the alpha-, beta-, and gammaproteobacteria and shares no significant identity with any riboflavin transporter previously identified. In this work, we show that RibN is localized in the membrane cellular fraction and its expression is downregulated by riboflavin. By heterologous expression in a Brucella abortus mutant auxotrophic for riboflavin, we demonstrate that RibN possesses flavin transport activity. Similarly, we also demonstrate that RibN orthologues from Ochrobactrum anthropi and Vibrio cholerae (which lacks the FMN riboswitch) are able to transport riboflavin. An R. leguminosarum ribN null mutant exhibited lower nodule occupancy levels in pea plants during symbiosis assays. Thus, we propose that RibN and its homologues belong to a novel family of riboflavin transporters. This work provides the first experimental description of riboflavin transporters in Gram-negative bacteria.
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