ArticleJournal of bacteriology2013
Nitrite reductase NirBD is induced and plays an important role during in vitro dormancy of Mycobacterium tuberculosis.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 62 citations in OpenAlex.
- The functional analysis of NirBD in microorganisms and the efficacies of NirBD on the soil nitrogen storage and NSynthetic and systems biotechnology · 2026Review
- A Key Metabolic Protein in Active Mycobacterium tuberculosis: Insights into Carbon, Nitrogen, and Sulfur Metabolism.Advances in experimental medicine and biology · 2026Review
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- Prominent transcriptomic changes in Mycobacterium intracellulare under acidic and oxidative stress.BMC genomics · 2024Article
- Orphan response regulator NnaR is critical for nitrate and nitrite assimilation inFrontiers in cellular and infection microbiology · 2024Article
- The PII protein interacts with the Amt ammonium transport and modulates nitrate/nitrite assimilation in mycobacteria.Frontiers in microbiology · 2024Article
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- Spatial and temporal conversion of nitrogen usingFrontiers in microbiology · 2023Article
- Delineating transcriptional crosstalk between Mycobacterium avium subsp. paratuberculosis and human THP-1 cells at the early stage of infection via dual RNA-seq analysis.Veterinary research · 2022Article
- Metabolomics Analysis for Nitrite Degradation by the Metabolites ofFoods (Basel, Switzerland) · 2022Article
- Subzero, saline incubations of Colwellia psychrerythraea reveal strategies and biomarkers for sustained life in extreme icy environments.Environmental microbiology · 2021Article
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- Insights from the comparative genome analysis of natural rubber degradingBioinformation · 2021Article
- Genomic Virulence Features of Two Novel SpeciesMicroorganisms · 2020Article
- Genome-wide identification of essential genes in Mycobacterium intracellulare by transposon sequencing - Implication for metabolic remodeling.Scientific reports · 2020Article
- TheProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Article
- Article
- Oxidative Phosphorylation as a Target Space for Tuberculosis: Success, Caution, and Future Directions.Microbiology spectrum · 2017Review
- The bacillary and macrophage response to hypoxia in tuberculosis and the consequences for T cell antigen recognition.Microbes and infection · 2017Review
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Mycobacterium tuberculosis is one of the strongest reducers of nitrate among all mycobacteria. Reduction of nitrate to nitrite, mediated by nitrate reductase (NarGHJI) of M. tuberculosis, is induced during the dormant stage, and the enzyme has a respiratory function in the absence of oxygen. Nitrite reductase (NirBD) is also functional during aerobic growth when nitrite is the sole nitrogen source. However, the role of NirBD-mediated nitrite reduction during the dormancy is not yet characterized. Here, we analyzed nitrite reduction during aerobic growth as well as in a hypoxic dormancy model of M. tuberculosis in vitro. When nitrite was used as the sole nitrogen source in the medium, the organism grew and the reduction of nitrite was evident in both hypoxic and aerobic cultures of M. tuberculosis. Remarkably, the hypoxic culture of M. tuberculosis, compared to the aerobic culture, showed 32- and 4-fold-increased expression of nitrite reductase (NirBD) at the transcription and protein levels, respectively. More importantly, a nirBD mutant of M. tuberculosis was unable to reduce nitrite and compared to the wild-type (WT) strain had a >2-log reduction in viability after 240 h in the Wayne model of hypoxic dormancy. Dependence of M. tuberculosis on nitrite reductase (NirBD) was also seen in a human macrophage-based dormancy model where the nirBD mutant was impaired for survival compared to the WT strain. Overall, the increased expression and essentiality of nitrite reductase in the in vitro dormancy models suggested that NirBD-mediated nitrite reduction could be critical during the persistent stage of M. tuberculosis.
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