ArticleApplied and environmental microbiology2013
Development of a tunable wide-range gene induction system useful for the study of streptococcal toxin-antitoxin systems.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 35 citations in OpenAlex.
- The HicAB System: Characteristics and Biological Roles of an Underappreciated Toxin-Antitoxin System.International journal of molecular sciences · 2024Review
- Construction of an arrayed CRISPRi library as a resource for essential gene function studies inMicrobiology spectrum · 2024Article
- Expanding the genetic toolbox for the obligate human pathogenFrontiers in bioengineering and biotechnology · 2024Article
- Illuminating the oral microbiome and its host interactions: tools and approaches for molecular microbiology studies.FEMS microbiology reviews · 2023Review
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- Human-associated bacteria adopt an unusual route for synthesizing 3-acetylated tetramates for environmental adaptation.Microbiome · 2023Article
- HicA Toxin-Based Counterselection Marker for Allelic Exchange Mutations in Fusobacterium nucleatum.Applied and environmental microbiology · 2023Article
- A tightly controlled gene induction system that contributes to the study of lethal gene function inJournal of oral microbiology · 2023Article
- Development of the First Tractable Genetic System for Parvimonas micra, a Ubiquitous Pathobiont in Human Dysbiotic Disease.Microbiology spectrum · 2022Article
- Post-translational modification of Streptococcus sanguinis SpxB influences protein solubility and HMolecular oral microbiology · 2021Article
- Characterization of FtsH Essentiality inFrontiers in genetics · 2021Article
- Repurposing the Streptococcus mutans CRISPR-Cas9 System to Understand Essential Gene Function.PLoS pathogens · 2020Article
- Spatial Correlations and Distribution of Competence Gene Expression in Biofilms ofFrontiers in microbiology · 2020Article
- Multiplex Imaging of Polymicrobial Communities-Murine Models to Study Oral Microbiome Interactions.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Article
- Examining the Protein Interactome and Subcellular Localization of RNase J2 Complexes inFrontiers in microbiology · 2019Article
- Identification of a Quorum Sensing System Regulating Capsule Polysaccharide Production and Biofilm Formation inFrontiers in cellular and infection microbiology · 2019Article
- Identification of New Degrons inFrontiers in microbiology · 2017Article
- Fine-tuned production of hydrogen peroxide promotes biofilm formation of Streptococcus parasanguinis by a pathogenic cohabitant Aggregatibacter actinomycetemcomitans.Environmental microbiology · 2016Article
- Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Despite the plethora of genetic tools that have been developed for use in Streptococcus mutans, the S. mutans genetic system still lacks an effective gene induction system exhibiting low basal expression and strong inducibility. Consequently, we created two hybrid gene induction cassettes referred to as Xyl-S1 and Xyl-S2. Both Xyl-S cassettes are xylose inducible and controlled by the Bacillus megaterium xylose repressor. The Xyl-S cassettes each demonstrated >600-fold-increased reporter activity in the presence of 1.2% (wt/vol) xylose. However, the Xyl-S1 cassette yielded a much higher maximum level of gene expression, whereas the Xyl-S2 cassette exhibited much lower uninduced basal expression. The cassettes also performed similarly in Streptococcus sanguinis and Streptococcus gordonii, which suggests that they are likely to be useful in a variety of streptococci. We demonstrate how both Xyl-S cassettes are particularly useful for the study of toxin-antitoxin (TA) modules using both the previously characterized S. mutans mazEF TA module and a previously uncharacterized HicAB TA module in S. mutans. HicAB TA modules are widely distributed among bacteria and archaea, but little is known about their function. We show that HicA serves as the toxin component of the module, while HicB serves as the antitoxin. Our results suggest that, in contrast to that of typical TA modules, HicA toxicity in S. mutans is modest at best. The implications of these results for HicAB function are discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.