ArticleApplied and environmental microbiology2013
Detection of Escherichia coli O157 by peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) and comparison to a standard culture method.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Peptide nucleic acid-based biosensor for detection ofNanomedicine (London, England) · 2026Article
- Rapid Bacteria Identification and Antimicrobial Susceptibility Testing via 5-Min Fluorescence In Situ Hybridization.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Peptide Nucleic Acid-Fluorescence In Situ Hybridization (PNA-FISH) for the Detection and Spatial Location of Microorganisms.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Nonculturable Escherichia coli O157 in horticultural compost: a public health concern.Access microbiology · 2025Article
- Antimicrobial Resistance Profile ofEnvironmental health insights · 2025Article
- Microfluidics combined with fluorescenceFrontiers in bioengineering and biotechnology · 2022Article
- Potential of Flow Cytometric Approaches for Rapid Microbial Detection and Characterization in the Food Industry-A Review.Foods (Basel, Switzerland) · 2021Review
- Establishment of a New PNA-FISH Method forMicroorganisms · 2020Article
- Genomic epidemiology and carbon metabolism of Escherichia coli serogroup O145 reflect contrasting phylogenies.PloS one · 2020Article
- Genetically Engineered Phages: a Review of Advances over the Last Decade.Microbiology and molecular biology reviews : MMBR · 2016Review
- Magnetic micro/nanoparticle flocculation-based signal amplification for biosensing.International journal of nanomedicine · 2016Article
- A novel enzyme-linked immunosorbent assay for detection of Escherichia coli O157:H7 using immunomagnetic and beacon gold nanoparticles.Gut pathogens · 2014Article
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7 authors.
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Abstract
Despite the emergence of non-O157 Shiga toxin-producing Escherichia coli (STEC) infections, E. coli serotype O157 is still the most commonly identified STEC in the world. It causes high morbidity and mortality and has been responsible for a number of outbreaks in many parts of the world. Various methods have been developed to detect this particular serotype, but standard bacteriological methods remain the gold standard. Here, we propose a new peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) method for the rapid detection of E. coli O157. Testing on 54 representative strains showed that the PNA probe is highly sensitive and specific to E. coli O157. The method then was optimized for detection in food samples. Ground beef and unpasteurized milk samples were artificially contaminated with E. coli O157 concentrations ranging from 1 × 10(-2) to 1 × 10(2) CFU per 25 g or ml of food. Samples were then preenriched and analyzed by both the traditional bacteriological method (ISO 16654:2001) and PNA-FISH. The PNA-FISH method performed well in both types of food matrices with a detection limit of 1 CFU/25 g or ml of food samples. Tests on 60 food samples have shown a specificity value of 100% (95% confidence interval [CI], 82.83 to 100), a sensitivity of 97.22% (95% CI, 83.79 to 99.85%), and an accuracy of 98.33% (CI 95%, 83.41 to 99.91%). Results indicate that PNA-FISH performed as well as the traditional culture methods and can reduce the diagnosis time to 1 day.
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