ArticlePlant molecular biology2014
NtERF32: a non-NIC2 locus AP2/ERF transcription factor required in jasmonate-inducible nicotine biosynthesis in tobacco.
Article in Plant molecular biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 86 citations in OpenAlex.
- Ethylene represses jasmonate signaling to attenuate nicotine biosynthesis inFrontiers in plant science · 2026Article
- AP2/ERF transcription factors regulate the biosynthesis of terpenoids, phenolics, and alkaloids in plants.Horticulture research · 2026Article
- Cloning of the DlERF10 gene from Diospyros lotus L. and cold tolerance analysis of the DlERF10 gene in transgenic tobacco plants.PloS one · 2025Article
- Genetic regulation and manipulation of nicotine biosynthesis in tobacco: strategies to eliminate addictive alkaloids.Journal of experimental botany · 2024Review
- Plant disease resistance outputs regulated by AP2/ERF transcription factor family.Stress biology · 2024Review
- Comparative transcriptome analysis reveals nicotine metabolism is a critical component for enhancing stress response intensity of innate immunity system in tobacco.Frontiers in plant science · 2024Article
- Citrus transcription factor CsERF1 is involved in the response to citrus tristeza disease.Frontiers in plant science · 2024Article
- Transcriptome and metabolome profiling of the medicinal plantFrontiers in genetics · 2023Article
- Regulation of the regulators: Transcription factors controlling biosynthesis of plant secondary metabolites during biotic stresses and their regulation by miRNAs.Frontiers in plant science · 2023Review
- Systematic Identification of Methyl Jasmonate-Responsive Long Noncoding RNAs and Their Nearby Coding Genes Unveils Their Potential Defence Roles in Tobacco BY-2 Cells.International journal of molecular sciences · 2022Article
- Interplay of transcription factors orchestrating the biosynthesis of plant alkaloids.3 Biotech · 2022Review
- Biosynthesis and the Roles of Plant Sterols in Development and Stress Responses.International journal of molecular sciences · 2022Review
- NtMYB305a binds to the jasmonate-responsive GAG region of NtPMT1a promoter to regulate nicotine biosynthesis.Plant physiology · 2022Article
- Transcription Factors in Alkaloid Engineering.Biomolecules · 2021Review
- Jasmonic acid and ERF family genes are involved in chilling sensitivity and seed browning of pepper fruit after harvest.Scientific reports · 2020Article
- Transcriptional Factors Regulate Plant Stress Responses through Mediating Secondary Metabolism.Genes · 2020Review
- Transcriptome analysis reveals key genes involved in the regulation of nicotine biosynthesis at early time points after topping in tobacco (Nicotiana tabacum L.).BMC plant biology · 2020Article
- MdMYC2 and MdERF3 Positively Co-Regulate α-Farnesene Biosynthesis in Apple.Frontiers in plant science · 2020Article
- Article
- A transcriptomic profile of topping responsive non-coding RNAs in tobacco roots (Nicotiana tabacum).BMC genomics · 2019Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nicotine biosynthesis in tobacco (Nicotiana tabacum L.) is highly regulated by jasmonic acid (JA). Two nuclear loci, A and B (renamed NIC1 and NIC2) have been identified that mediate JA-inducible nicotine formation and total alkaloid accumulation. NIC2 was recently shown to be a cluster of seven genes encoding Apetala2/Ethylene-Response Factor (AP2/ERF)-domain transcription factors (TFs) in Group IX of the tobacco AP2/ERF family. Here we report the characterization of several NtERF TF genes that are not within the NIC2 locus, but required for methyl JA (MeJA)-induced nicotine biosynthesis. Expression of NtERF1, NtERF32, and NtERF121 is rapidly induced (<30 min) by MeJA treatment. All three of these TFs specifically bind the GCC box-like element of the GAG motif required for MeJA-induced transcription of NtPMT1a, a gene encoding putrescine N-methyltransferase, the first committed step in the synthesis of the nicotine pyrrolidine ring. Ectopic overexpression of NtERF32 increases expression of NtPMT1a in vivo and elevates total alkaloid contents, whereas RNAi-mediated knockdown of NtERF32 reduces the mRNA levels of multiple genes in the nicotine biosynthetic pathway including NtPMT1a and quinolinate phosphoribosyltransferase (NtQPT2), and lowers nicotine and total alkaloid levels. We conclude that NtERF32 and related ERF genes are important non-NIC2 locus associated transcriptional regulators of nicotine and total alkaloid formation.
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