ArticleBiomaterials2013
Non-invasive monitoring of cell metabolism and lipid production in 3D engineered human adipose tissues using label-free multiphoton microscopy.
Article in Biomaterials, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed.
- Submicromolar imaging of intrinsic chromophores by two-photon photothermal microscopy captures mitochondrial response to chemotherapy.Science advances · 2026Article
- Label-Free Optical Metabolic Imaging in Cells and Tissues.Annual review of biomedical engineering · 2023Review
- Bioorthogonal Chemical Imaging of Cell Metabolism Regulated by Aromatic Amino Acids.Journal of visualized experiments : JoVE · 2023Article
- Ultrafast fluorescence dynamics of NADH in aprotic solvents: Quasi-static self-quenching unmasked.Journal of photochemistry and photobiology. A, Chemistry · 2023Article
- Light distribution in fat cell layers at physiological temperatures.Scientific reports · 2023Article
- Coordinated metabolic transitions and gene expression by NAD+ during adipogenesis.The Journal of cell biology · 2022Article
- Label-free third harmonic generation imaging and quantification of lipid droplets in live filamentous fungi.Scientific reports · 2022Article
- Article
- Tracking Photodynamic- and Chemotherapy-Induced Redox-State Perturbations in 3D Culture Models of Pancreatic Cancer: A Tool for Identifying Therapy-Induced Metabolic Changes.Journal of clinical medicine · 2019Article
- Evaluating Cell Metabolism Through Autofluorescence Imaging of NAD(P)H and FAD.Antioxidants & redox signaling · 2019Review
- 3D Reconstruction of Lipid Droplets in the Seed of Brassica napus.Scientific reports · 2018Article
- Multicolor two-photon imaging of endogenous fluorophores in living tissues by wavelength mixing.Scientific reports · 2017Article
- Tissue-Engineered Solutions in Plastic and Reconstructive Surgery: Principles and Practice.Frontiers in surgery · 2017Review
- Non-invasive Assessments of Adipose Tissue Metabolism In Vitro.Annals of biomedical engineering · 2016Review
- Optical methods for quantitative and label-free sensing in living human tissues: principles, techniques, and applications.Advances in physics · 2016Article
- Strategies for improving the physiological relevance of human engineered tissues.Trends in biotechnology · 2015Review
- Imaging strategies for tissue engineering applications.Tissue engineering. Part B, Reviews · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
Non-linear optical microscopy methods can characterize over time multiple functional properties of engineered tissues during development. Here, we demonstrate how the combined use of third-harmonic generation (THG) and two-photon excited fluorescence (2PEF) imaging can provide direct quantitative biomarkers of adipogenic stem cell differentiation and metabolic state, respectively. Specifically, we imaged over nine weeks silk scaffolds embedded with human mesenchymal stem cells and exposed to either propagation (PM) or adipogenic differentiation media (AM). THG was employed to visualize the formation of lipid droplets. 2PEF was used to assess the metabolic state of the cells through the redox ratio defined based on the endogenous FAD and NADH fluorescence. The redox ratio of cells in the AM scaffold was significantly lower than that in the PM scaffold during week 5 and 9, and correlated with significant increases in lipid-to-cell volume ratio, and number and size of lipid droplets in the AM scaffold. These findings indicate that the decrease in redox ratio during adipogenic differentiation is associated with fatty acid synthesis and lipid accumulation. Our methods therefore enabled us to identify and measure dynamic correlations between lipid droplet formation and cell metabolic state, while providing insight on the spatial heterogeneity of the observed signals.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.