ReviewMethods in cell biology2013
Circumventing photodamage in live-cell microscopy.
Review in Methods in cell biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 112 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
112 citing papers in PubMed, 1 synthesis or guideline pooled it.
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- Label-Free All-Electrical Tracking of Individual and Collective Cell Migration on a Megapixel CMOS Capacitance Sensor.bioRxiv : the preprint server for biology · 2026Article
- Live-cell STED microscopy enables 50 nm resolution imaging with preserved cell proliferation.Scientific reports · 2026Article
- Intravital mesoscale optical imaging: challenges, techniques, and future perspectives.Biophysics reports · 2026Article
- A Versatile Three Dimensional Traction Force Microscopy Framework for Uncovering the Mechanics of Bio-Adhesion.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Nuclear Dynamics and Its Timing Regulation Revealed by Live-Cell Imaging.Advances in experimental medicine and biology · 2026Review
- Methods to analyze cell migration data: fundamentals and practical guidelines.Nature methods · 2026Review
- Hyper-spectral imaging with up-converted mid-infrared single-photons.Scientific reports · 2025Article
- Quantitative imaging of individual bacterial cells:Journal of biomedical optics · 2025Article
- Standardized measurements for monitoring and comparing multiphoton microscope systems.Nature protocols · 2025Review
- Spatial microfluidic holographic integrated platform for label-free and high-dimensional analysis of cancer heterogeneity.Nature communications · 2025Article
- A data-driven model for mitochondrial inner membrane remodeling as a driving force of organelle shaping.Journal of cell science · 2025Article
- Distinct actin microfilament localization during early cell plate formation through deep learning-based image restoration.Plant cell reports · 2025Article
- Ultrabright and ultrafast afterglow imaging in vivo via nanoparticles made of trianthracene derivatives.Nature biomedical engineering · 2025Article
- Deep learning image analysis for continuous single-cell imaging of dynamic processes in Plasmodium falciparum-infected erythrocytes.Communications biology · 2025Article
- Pore morphology of bijel-templated materials promotes migration and downregulates αSMA expression in human fibroblasts.Frontiers in bioengineering and biotechnology · 2025Article
52 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
Fluorescence microscopy has become an essential tool in cell biology. This technique allows researchers to visualize the dynamics of tissue, cells, individual organelles, and macromolecular assemblies inside the cell. Unfortunately, fluorescence microscopy is not completely "noninvasive" as the high-intensity excitation light required for excitation of fluorophores is inherently toxic for live cells. Physiological changes induced by excessive illumination can lead to artifacts and abnormal responses. In this chapter, we review major factors that contribute to phototoxicity and discuss practical solutions for circumventing photodamage. These solutions include the proper choice of image acquisition parameters, optimization of filter sets, hardware synchronization, and the use of intelligent illumination to avoid unnecessary light exposure.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.