ArticleThe journal of physical chemistry. B2013
DNA adsorption to and elution from silica surfaces: influence of amino acid buffers.
Article in The journal of physical chemistry. B, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 54 citations in OpenAlex.
- Quantitative analysis of sequential nucleic acid elution from silica paramagnetic beads.The Analyst · 2026Article
- Implementation of a high throughput automated platform for residual DNA quantitation.PloS one · 2025Article
- Recent advances in RNA sample preparation techniques for the detection of SARS-CoV-2 in saliva and gargle.Trends in analytical chemistry : TRAC · 2023Review
- Electrospun Scaffolds Enriched with Nanoparticle-Associated DNA: General Properties, DNA Release and Cell Transfection.Polymers · 2023Article
- UV-Assisted Hyperbranched Poly(β-amino ester) Modification of a Silica Membrane for Two-Step Microfluidic DNA Extraction from Blood.ACS applied materials & interfaces · 2023Article
- Integrated "lab-on-a-chip" microfluidic systems for isolation, enrichment, and analysis of cancer biomarkers.Lab on a chip · 2023Review
- Improving Yields in Multi-analyte Extractions by Utilizing Post-homogenized Tissue Debris.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2023Article
- Simple and Economical Extraction of Viral RNA and Storage at Ambient Temperature.Microbiology spectrum · 2022Article
- The Multiple States of Environmental DNA and What Is Known about Their Persistence in Aquatic Environments.Environmental science & technology · 2022Review
- Cascade Filtration With PCR Detection and Field-Flow-Fractionation Online With ICP-MS for the Characterization of DNA Interaction With Suspended Particulate Matter.Frontiers in chemistry · 2022Article
- Article
- High-yield purification of exceptional-quality, single-molecule DNA substrates.Journal of biological methods · 2021Article
- Adsorption and desorption of DNA-functionalized beads in glass microfluidic channels.Biomicrofluidics · 2019Article
- Enhanced solid phase extraction of DNA using hydrophilic monodisperse poly(methacrylic acid-co-ethylene dimethacrylate) microparticles.Molecular biology reports · 2019Article
- Charge-Dependent Regulation in DNA Adsorption on 2D Clay Minerals.Scientific reports · 2019Article
- DNA Delivery Systems Based on Peptide-Mimicking Cationic Lipids-The Effect of the Co-Lipid on the Structure and DNA Binding Capacity.Langmuir : the ACS journal of surfaces and colloids · 2019Article
- Methods to Investigate the Global Atmospheric Microbiome.Frontiers in microbiology · 2019Article
- Extraction of microRNAs from biological matrices with titanium dioxide nanofibers.Analytical and bioanalytical chemistry · 2018Article
- Synthesis of Silica-coated Iron Oxide Nanoparticles: Preventing Aggregation without Using Additives or Seed Pretreatment.Iranian journal of pharmaceutical research : IJPR · 2018Article
- Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Solid phase extraction and purification of DNA from complex samples typically requires chaotropic salts that can inhibit downstream polymerase amplification if carried into the elution buffer. Amino acid buffers may serve as a more compatible alternative for modulating the interaction between DNA and silica surfaces. We characterized DNA binding to silica surfaces, facilitated by representative amino acid buffers, and the subsequent elution of DNA from the silica surfaces. Through bulk depletion experiments, we found that more DNA adsorbs to silica particles out of positively compared to negatively charged amino acid buffers. Additionally, the type of the silica surface greatly influences the amount of DNA adsorbed and the final elution yield. Quartz crystal microbalance experiments with dissipation monitoring (QCM-D) revealed multiphasic DNA adsorption out of stronger adsorbing conditions such as arginine, glycine, and glutamine, with DNA more rigidly bound during the early stages of the adsorption process. The DNA film adsorbed out of glutamate was more flexible and uniform throughout the adsorption process. QCM-D characterization of DNA elution from the silica surface indicates an uptake in water mass during the initial stage of DNA elution for the stronger adsorbing conditions, which suggests that for these conditions the DNA film is partly dehydrated during the prior adsorption process. Overall, several positively charged and polar neutral amino acid buffers show promise as an alternative to methods based on chaotropic salts for solid phase DNA extraction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.