ArticleBiophysical journal2013
Mechanism of how salt-gradient-induced charges affect the translocation of DNA molecules through a nanopore.
Article in Biophysical journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Improving the Performance of Selective Solid-State Nanopore Sensing Using a Polyhistidine-Tagged Monovalent Streptavidin.ACS sensors · 2024Article
- DNA translocation through pH-dependent soft nanopores.European biophysics journal : EBJ · 2021Article
- Slowing down DNA translocation velocity using a LiCl salt gradient and nanofiber mesh.European biophysics journal : EBJ · 2019Article
- Electrokinetic Analysis of Energy Harvest from Natural Salt Gradients in Nanochannels.Scientific reports · 2017Article
- Effects of Polymer Length and Salt Concentration on the Transport of ssDNA in Nanofluidic Channels.Biophysical journal · 2017Article
- Investigating asymmetric salt profiles for nanopore DNA sequencing with biological porin MspA.PloS one · 2017Article
- The Nucleotide Capture Region of Alpha Hemolysin: Insights into Nanopore Design for DNA Sequencing from Molecular Dynamics Simulations.Nanomaterials (Basel, Switzerland) · 2015Article
- Biomedical diagnosis perspective of epigenetic detections using alpha-hemolysin nanopore.AIMS materials science · 2015Article
- Smooth DNA transport through a narrowed pore geometry.Biophysical journal · 2014Article
- DNA capture and translocation through nanoscale pores-a fine balance of electrophoresis and electroosmosis.Biophysical journal · 2013Article
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6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Experiments using nanopores demonstrated that a salt gradient enhances the capture rate of DNA and reduces its translocation speed. These two effects can help to enable electrical DNA sequencing with nanopores. Here, we provide a quantitative theoretical evaluation that shows the positive net charges, which accumulate around the pore entrance due to the salt gradient, are responsible for the two observed effects: they reinforce the electric capture field, resulting in promoted molecule capture rate; and they induce cationic electroosmotic flow through the nanopore, thus significantly retarding the motion of the anionic DNA through the nanopore. Our multiphysical simulation results show that, during the polymer trapping stage, the former effect plays the major role, thus resulting in promoted DNA capture rate, while during the nanopore-penetrating stage the latter effect dominates and consequently reduces the DNA translocation speed significantly. Quantitative agreement with experimental results has been reached by further taking nanopore wall surface charges into account.
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