ArticleBiophysical journal2013
Quantification of biomass and cell motion in human pluripotent stem cell colonies.
Article in Biophysical journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Increased mitochondrial mutation heteroplasmy induces aging phenotypes in pluripotent stem cells and their differentiated progeny.Aging cell · 2025Article
- LVING reveals the intracellular structure of cell growth.Scientific reports · 2024Article
- Fabrication and validation of an LED array microscope for multimodal, quantitative imaging.HardwareX · 2023Article
- Review
- Microfluidic Single-Cell Analytics.Advances in biochemical engineering/biotechnology · 2022Article
- Epigenetic Reprogramming of the Glucose Metabolic Pathways by the Chromatin Effectors During Cancer.Sub-cellular biochemistry · 2022Article
- Viscoelasticity, Like Forces, Plays a Role in Mechanotransduction.Frontiers in cell and developmental biology · 2022Review
- Article
- A Rainbow Reporter Tracks Single Cells and Reveals Heterogeneous Cellular Dynamics among Pluripotent Stem Cells and Their Differentiated Derivatives.Stem cell reports · 2020Article
- Cell viscoelasticity is linked to fluctuations in cell biomass distributions.Scientific reports · 2020Article
- The recent advances in the mathematical modelling of human pluripotent stem cells.SN applied sciences · 2020Review
- Identifying fates of cancer cells exposed to mitotic inhibitors by quantitative phase imaging.The Analyst · 2019Article
- Topological Arrangement of Cardiac Fibroblasts Regulates Cellular Plasticity.Circulation research · 2018Article
- Soft lithography fabrication of index-matched microfluidic devices for reducing artifacts in fluorescence and quantitative phase imaging.Microfluidics and nanofluidics · 2018Article
- Living cell dry mass measurement using quantitative phase imaging with quadriwave lateral shearing interferometry: an accuracy and sensitivity discussion.Journal of biomedical optics · 2015Article
- Live-cell mass profiling: an emerging approach in quantitative biophysics.Nature methods · 2014Article
- Live cell interferometry quantifies dynamics of biomass partitioning during cytokinesis.PloS one · 2014Article
- Fast and furious: the mass and motion of stem cells.Biophysical journal · 2013Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Somatic cell reprogramming to pluripotency requires an immediate increase in cell proliferation and reduction in cell size. It is unknown whether proliferation and biomass controls are similarly coordinated with early events during the differentiation of pluripotent stem cells (PSCs). This impasse exists because PSCs grow in tight clusters or colonies, precluding most quantifying approaches. Here, we investigate live cell interferometry as an approach to quantify the biomass and growth of HSF1 human PSC colonies before and during retinoic acid-induced differentiation. We also provide an approach for measuring the rate and coordination of intracolony mass redistribution in HSF1 clusters using live cell interferometry images. We show that HSF1 cells grow at a consistent, exponential rate regardless of colony size and display coordinated intracolony movement that ceases with the onset of differentiation. By contrast, growth and proliferation rates show a decrease of only ∼15% decrease during early differentiation despite global changes in gene expression and previously reported changes in energy metabolism. Overall, these results suggest that cell biomass and proliferation are regulated independent of pluripotency during early differentiation, which is distinct from what occurs with successful reprogramming.
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Registered trials
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