ArticleVector borne and zoonotic diseases (Larchmont, N.Y.)2013
Mosquito protein kinase G phosphorylates flavivirus NS5 and alters flight behavior in Aedes aegypti and Anopheles gambiae.
Article in Vector borne and zoonotic diseases (Larchmont, N.Y.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- Phosphorylation landscape of dengue virus proteins and their implications in protein-protein interactions.PloS one · 2026Article
- CK1 and PP1 regulate Rift Valley fever virus genome replication through L protein phosphorylation.Antiviral research · 2024Article
- Circadian and daily rhythms of disease vector mosquitoes.Current opinion in insect science · 2024Review
- Circadian regulation of dengue virus transmission and replication: insights into vector activity and viral dynamics.Frontiers in cellular and infection microbiology · 2024Review
- Influence of RVFV Infection on Olfactory Perception and Behavior inPathogens (Basel, Switzerland) · 2023Article
- Rift Valley fever virus detection in susceptible hosts with special emphasis in insects.Scientific reports · 2021Article
- Vertical Transmission of Zika Virus (Flaviviridae, Flavivirus) in Amazonian Aedes aegypti (Diptera: Culicidae) Delays Egg Hatching and Larval Development of Progeny.Journal of medical entomology · 2019Article
- Suppression of Type I Interferon Signaling byViruses · 2018Review
- Keeping track of mosquitoes: a review of tools to track, record and analyse mosquito flight.Parasites & vectors · 2018Review
- Review
- Decreased Flight Activity in Culex pipiens (Diptera: Culicidae) Naturally Infected With Culex flavivirus.Journal of medical entomology · 2016Article
- West Nile virus methyltransferase domain interacts with protein kinase G.Virology journal · 2013Article
- Extensive circadian and light regulation of the transcriptome in the malaria mosquito Anopheles gambiae.BMC genomics · 2013Article
- Daily rhythms in antennal protein and olfactory sensitivity in the malaria mosquito Anopheles gambiae.Scientific reports · 2013Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
Many arboviral proteins are phosphorylated in infected mammalian cells, but it is unknown if the same phosphorylation events occur when insects are similarly infected. One of the mammalian kinases responsible for phosphorylation, protein kinase G (PKG), has been implicated in the behavior of multiple nonvector insects, but is unstudied in mosquitoes. PKG from Aedes aegypti was cloned, and phosphorylation of specific viral sites was monitored by mass spectrometry from biochemical and cell culture experiments. PKG from Aedes mosquitoes is able to phosphorylate dengue nonstructural protein 5 (NS5) at specific sites in cell culture and cell-free systems and autophosphorylates its own regulatory domain in a cell-free system. Injecting Aedes aegypti and Anopheles gambiae mosquitoes with a pharmacological PKG activator resulted in increased Aedes wing activity during periods of their natural diurnal/crepuscular activity and increased Anopheles nocturnal locomotor/flight activity. Thus, perturbation of the PKG signaling pathway in mosquitoes alters flight behavior. The demonstrated effect of PKG alterations is consistent with a viral PKG substrate triggering increased PKG activity. This increased PKG activity could be the mechanism by which dengue virus increases flight behavior and possibly facilitates transmission. Whether or not PKG is part of the mechanism by which dengue increases flight behavior, this report is the first to show PKG can modulate behavior in hematophagous disease vectors.
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