ArticlePharmacognosy magazine2013
Identification of ethnomedicinal plants (Rauvolfioideae: Apocynaceae) through DNA barcoding from northeast India.
Article in Pharmacognosy magazine, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 26 citations in OpenAlex.
- Conservation Status, Plastome Diversity, and Evolutionary Diversification of Three ArabianBiology · 2026Article
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- DNA Barcoding Medicinal Plant Species from Indonesia.Plants (Basel, Switzerland) · 2022Article
- First genomic insights into theFrontiers in plant science · 2022Article
- ITS2: An Ideal DNA Barcode for the Arid Medicinal Plant Rhazya Stricta.Pharmaceutical medicine · 2019Article
- DNA barcoding for the efficient and accurate identification of medicinal polygonati rhizoma in China.PloS one · 2018Article
- Efficiency of matK, rbcL, trnH-psbA, and trnL-F (cpDNA) to Molecularly Authenticate Philippine Ethnomedicinal Apocynaceae Through DNA Barcoding.Pharmacognosy magazine · 2016Article
- The Use of DNA Barcoding on Recently Diverged Species in the Genus Gentiana (Gentianaceae) in China.PloS one · 2016Article
- Application of Partial Internal Transcribed Spacer Sequences for the Discrimination of Artemisia capillaris from Other Artemisia Species.Evidence-based complementary and alternative medicine : eCAM · 2016Article
- Utility of indels for species-level identification of a biologically complex plant group: a study with intergenic spacer in Citrus.Molecular biology reports · 2014Article
- Evaluating the Feasibility of Five Candidate DNA Barcoding Loci for PhilippinePharmacognosy magazineArticle
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3 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundDNA barcode-based molecular characterization is in practice for plants, but yet lacks total agreement considering the selection of marker. Plant species of subfamily Rauvolfioideae have long been used as herbal medicine by the majority of tribal people in Northeast (NE) India and at present holds mass effect on the society. Hence, there is an urgent need of correct taxonomic inventorization vis-à-vis species level molecular characterization of important medicinal plants.
objectiveTo test the efficiency of matK in species delineation like DNA barcoding in Rauvolfiadae (Apocynaceae). MATERIALS AND
methodsIn this study, the core DNA barcode matK and trnH-psbA sequences are examined for differentiation of selected ethnomedicinal plants of Apocynaceae. DNA from young leaves of selected species was isolated, and matK gene (~800 bp) and trnH-psbA spacer (~450 bp) of Chloroplast DNA was amplified for species level identification.
resultsThe ~758 bp matK sequence in comparison to the trnH-psbA showed easy amplification, alignment, and high level of discrimination value among the medicinal Rauvolfioidae species. Intergenic spacer trnH-psbA is also exhibited persistent problem in obtaining constant bidirectional sequences. Partial matK sequences exhibited 3 indels in multiple of 3 at 5 end. Evidently, generated matK sequences are clustered cohesively, with their conspecific Genbank sequences. However, repeat structures with AT-rich regions, possessing indels in multiple of 3, could be utilized as qualitative molecular markers in further studies both at the intra-specific and shallow inter-specific levels like the intergenic spacers of CpDNA.
conclusionmatK sequence information could help in correct species identification for medicinal plants of Rauvolfioideae.
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