ArticleJournal of biological rhythms2013
E and M circadian pacemaker neurons use different PDF receptor signalosome components in drosophila.
Article in Journal of biological rhythms, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Neuropeptide dynamics coordinate layered plasticity mechanisms adaptingScience advances · 2025Article
- Mutual coupling of neurons in the circadian master clock: What we can learn from fruit flies.Neurobiology of sleep and circadian rhythms · 2025Article
- Genomic Diversity Illuminates the Environmental Adaptation of Drosophila suzukii.Genome biology and evolution · 2024Article
- Article
- Discovery of levodopa-induced dyskinesia-associated genes using genomic studies in patients and Drosophila behavioral analyses.Communications biology · 2022Article
- Regulation of PDF receptor signaling controlling daily locomotor rhythms in Drosophila.PLoS genetics · 2022Article
- High-Frequency Neuronal Bursting is Essential for Circadian and Sleep Behaviors inThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Regulation of Olfactory Associative Memory by the Circadian Clock Output Signal Pigment-Dispersing Factor (PDF).The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Molecular and circuit mechanisms mediating circadian clock output in the Drosophila brain.The European journal of neuroscience · 2020Review
- Peptidergic signaling from clock neurons regulates reproductive dormancy in Drosophila melanogaster.PLoS genetics · 2019Article
- A Symphony of Signals: Intercellular and Intracellular Signaling Mechanisms Underlying Circadian Timekeeping in Mice and Flies.International journal of molecular sciences · 2019Review
- Coordination between Differentially Regulated Circadian Clocks Generates Rhythmic Behavior.Cold Spring Harbor perspectives in biology · 2018Review
- Membrane Currents, Gene Expression, and Circadian Clocks.Cold Spring Harbor perspectives in biology · 2017Review
- Functional PDF Signaling in the Drosophila Circadian Neural Circuit Is Gated by Ral A-Dependent Modulation.Neuron · 2016Article
- Model Organisms in G Protein-Coupled Receptor Research.Molecular pharmacology · 2015Review
- Pigment-Dispersing Factor Signaling and Circadian Rhythms in Insect Locomotor Activity.Current opinion in insect science · 2014Article
- The Drosophila neuropeptides PDF and sNPF have opposing electrophysiological and molecular effects on central neurons.Journal of neurophysiology · 2014Article
- Signaling of pigment-dispersing factor (PDF) in the Madeira cockroach Rhyparobia maderae.PloS one · 2014Article
- Article
- Rhythmic control of activity and sleep by class B1 GPCRs.Critical reviews in biochemistry and molecular biologyReview
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2 authors.
Funding
Abstract
We used real-time imaging to detect cAMP levels in neurons of intact fly brains to study the mechanisms of circadian pacemaker synchronization by the neuropeptide pigment dispersing factor (PDF) in Drosophila. PDF receptor (PDF-R) is expressed by both M (sLNv) and E (LNd) pacemaker subclasses and is coupled to G(sα) in both cases. We previously reported that PDF-R in M pacemakers elevates cAMP levels by activating the ortholog of mammalian adenylate cyclase 3 (AC3) but that AC3 disruptions had no effect on E pacemaker sensitivity to PDF. Here, we show that PDF-R in E pacemakers activates a different AC isoform, AC78C, an ortholog of mammalian AC8. Knockdown of AC78C by transgenic RNAi substantially reduces, but does not completely abrogate, PDF responses in these E pacemakers. The knockdown effect is intact when restricted to mature stages, suggesting a physiological and not a development role for AC78C in E pacemakers. The AC78C phenotype is rescued by the overexpression of AC78C but not by overexpression of the rutabaga AC. AC78C overexpression does not disrupt PDF responses in these E pacemakers, and neither AC78C knockdown nor its overexpression disrupted locomotor rhythms. Finally, knockdown of 2 AKAPs, nervy and AKAP200, partially reduces LNd PDF responses. These findings begin to identify the components of E pacemaker PDF-R signalosomes and indicate that they are distinct from PDF-R signalosomes in M pacemakers: we propose they contain AC78C and at least 1 other AC.
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