ArticleMethods in molecular biology (Clifton, N.J.)2013
Single-neuron transcriptome and methylome sequencing for epigenomic analysis of aging.
Article in Methods in molecular biology (Clifton, N.J.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed.
- Animal models in neuroscience with alternative approaches: Evolutionary, biomedical, and ethical perspectives.Animal models and experimental medicine · 2024Review
- DNA Methylation Machinery in Gastropod Mollusks.Life (Basel, Switzerland) · 2024Review
- RNA Isolation from Ctenophores.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Aplysia Neurons as a Model of Alzheimer's Disease: Shared Genes and Differential Expression.Journal of molecular neuroscience : MN · 2022Article
- ATP signaling in the integrative neural center of Aplysia californica.Scientific reports · 2021Article
- Co-expression analysis identifies neuro-inflammation as a driver of sensory neuron aging in Aplysia californica.PloS one · 2021Article
- A versatile depigmentation, clearing, and labeling method for exploring nervous system diversity.Science advances · 2020Article
- Changes in Metabolism and Proteostasis Drive Aging Phenotype inFrontiers in aging neuroscience · 2020Article
- Low concentrations of Lactobacillus rhamnosus GG (YobaBMC research notes · 2019Article
- Dicyemida and Orthonectida: Two Stories of Body Plan Simplification.Frontiers in genetics · 2019Article
- Identification of differential gene expression in endothelial cells from young and aged mice using RNA-Seq technique.American journal of translational research · 2019Article
- Intermediate-term memory inLearning & memory (Cold Spring Harbor, N.Y.) · 2018Article
- Chromatin Changes Associated with Neuronal Maintenance and Their Pharmacological Application.Current neuropharmacology · 2018Review
- Computational approaches for interpreting scRNA-seq data.FEBS letters · 2017Review
- Effect of cadmium on cytosine hydroxymethylation in gastropod hepatopancreas.Environmental science and pollution research international · 2017Article
- High-level dietary cadmium exposure is associated with global DNA hypermethylation in the gastropod hepatopancreas.PloS one · 2017Article
- Single-cell genomics: coming of age.Genome biology · 2016Article
- Cell type-specific gene expression profiling in brain tissue: comparison between TRAP, LCM and RNA-seq.BMB reports · 2015Article
- Single-cell analysis of the transcriptome and its application in the characterization of stem cells and early embryos.Cellular and molecular life sciences : CMLS · 2014Review
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Authors and funding
2 authors.
Funding
Abstract
Enormous heterogeneity in transcription and signaling is the feature that slows down progress in our understanding of the mechanisms of normal aging and age-related diseases. This is critical for neurobiology of aging where the enormous diversity of neuronal populations presents a significant challenge in experimental design. Here, we introduce Aplysia as a model for genomic analysis of aging at the single-cell level and provide protocols for integrated transcriptome and methylome profiling of individually identified neurons during the aging process. These single-cell RNA-seq and DNA methylation assays (methyl-capture/methyl enrichment) are compatible with all major next generation sequencing platforms (we used Roche/454 and SOLiD/Life Technologies as illustrative examples) and can be used to integrate an epigenetic signature with transcriptional output. The described sequencing library construction protocol provides both quantitative and directional information from transcriptional profiling of individual cells. Our results also confirm that different copies of DNA in polyploid Aplysia neurons behave similarly with respect to their DNA methylation.
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