ArticleJournal of reproduction & infertility2010
Effects of Timing on Cell Biopsy from Pre-compacted Morula Stage Bovine Embryos on Subsequent Embryonic Development.
Article in Journal of reproduction & infertility, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
3 citing papers in PubMed, 7 citations in OpenAlex.
- A pre-breeding screening program for transgenic boars based on fluorescence in situ hybridization assay.Transgenic research · 2014Article
- Article
- The effect of biopsy during precompacted morula stage on post vitrification development of blastocyst derived bovine embryos.Avicenna journal of medical biotechnology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionEmbryo biopsy has potential applications in molecular research processes in domestic animals, besides its application in sex determination in embryo transfer programs. The objective of the present study was to assess the in vitro development of bovine embryos biopsied on different days of precompacted morula stage. MATERIALS AND
methodsSlaughterhouse-derived oocytes were matured in vitro, fertilized (Day-0) by frozen-thawed, Percol-separated spermatozoa and cultured on oviductal cell monolayer. The embryos were subjected to cell biopsy on Days 2, 3, and 4 postinsemination at 4-16-cell stages. The data were analyzed using ANOVA and Chi-squared tests (SigmaStat, version 2). A p-value < 0.05 was considered significant.
resultsBiopsies carried out at 16-cell stage (Day-4) resulted in 94% of embryos developing to the blastocyst stage, which was significantly higher (p < 0.05) than the ones biopsied at 8-cell stage on Day-4 (64%), and those undergoing the procedure on Day-3 (49% and 46% at 4-cell and 8-cell stages, respectively) and Day-2 (39% and 33% at 4-cell and 8-cell stages, respectively). No significant differences were observed between biopsied and non-biopsied embryos on a given day. The total cell number in biopsy-derived blastocysts ranged between 103 and 135. The difference in the number of total cells, dead cells and cell allocation to trophectoderm and inner cell mass between non-biopsied and biopsy-derived blastocysts was insignificant.
conclusionBiopsy of bovine embryos at 4-16-cell stages had no adverse effects on in vitro developmental potentials and the 16-cell stage embryos, biopsied on Day-4 was the best stage for blastomere removal.
Indexed as
Identifiers
23926477PMC3719275W2154685924What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.