ArticleJournal of immunology (Baltimore, Md. : 1950)2013
Identification of a phage-encoded Ig-binding protein from invasive Neisseria meningitidis.
Article in Journal of immunology (Baltimore, Md. : 1950), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 33 citations in OpenAlex.
- A viral glycoprotein targets IgGEMBO molecular medicine · 2026Article
- Inactivation of the porB gene reduces the virulence of Neisseria meningitidis in transgenic mice.BMC microbiology · 2025Article
- First Characterization ofViruses · 2024Article
- Genetic variants linked to the phenotypic outcome of invasive disease and carriage ofMicrobial genomics · 2023Article
- Peptide barcode of multidrug-resistant strains of Neisseria gonorrhoeae isolated from patients in Thailand.PloS one · 2023Article
- Comprehensive Genome Analysis ofInternational journal of molecular sciences · 2022Article
- Bacteriophage and Bacterial Susceptibility, Resistance, and Tolerance to Antibiotics.Pharmaceutics · 2022Review
- Deconstructing the Phage-Bacterial Biofilm Interaction as a Basis to Establish New Antibiofilm Strategies.Viruses · 2022Review
- Genome-wide association studies reveal the role of polymorphisms affecting factor H binding protein expression in host invasion by Neisseria meningitidis.PLoS pathogens · 2021Article
- Interactions of Bacteriophages with Animal and Human Organisms-Safety Issues in the Light of Phage Therapy.International journal of molecular sciences · 2021Review
- Neisseria meningitidis: using genomics to understand diversity, evolution and pathogenesis.Nature reviews. Microbiology · 2020Review
- Acquisition of virulence genes by a carrier strain gave rise to the ongoing epidemics of meningococcal disease in West Africa.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Enrichment of high affinity subclasses and glycoforms from serum-derived IgG using FcγRs as affinity ligands.Biotechnology and bioengineering · 2018Article
- Is Genetic Mobilization Considered When Using Bacteriophages in Antimicrobial Therapy?Antibiotics (Basel, Switzerland) · 2017Article
- A virulence-associated filamentous bacteriophage of Neisseria meningitidis increases host-cell colonisation.PLoS pathogens · 2017Article
- Phages in the Human Body.Frontiers in microbiology · 2017Review
- Biological Functions of the Secretome ofFrontiers in cellular and infection microbiology · 2017Review
- Resistance of Neisseria meningitidis to human serum depends on T and B cell stimulating protein B.Infection and immunity · 2015Article
- Article
- CMV-encoded Fcγ receptors: modulators at the interface of innate and adaptive immunity.Seminars in immunopathology · 2014Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Ig-binding proteins are employed by a variety of organisms to evade the immune system. To our knowledge, we now report for the first time that meningococcal strains from several capsular groups exhibit Ig-binding activity that is dependent on human serum factors. A protein mediating Ig binding was identified as T and B cell-stimulating protein B (TspB) by immunoprecipitation and by mass spectroscopic analysis of tryptic peptides. Recombinant TspB and derivatives verified Ig binding, with a preference for human IgG2 Fc, and localized the IgG-binding region to a highly conserved subdomain of TspB. Antiserum produced in mice against the conserved subdomain detected the presence of TspB on the cell surface by flow cytometry when bacteria were grown in the presence of human serum. By fluorescence microscopy, we observed formation of an extracellular matrix having characteristics of a biofilm containing TspB, human IgG, DNA, and large aggregates of bacteria. TspB is encoded by gene ORF6 in prophage DNA, which others have shown is associated with invasive meningococcal strains. Knocking out ORF6 genes eliminated IgG binding and formation of large bacterial aggregates in biofilm. Reintroduction of a wild-type ORF6 gene by phage transduction restored the phenotype. The results show that TspB mediated IgG binding and aggregate/biofilm formation triggered by factors in human serum. As has been observed for other Ig-binding proteins, the activities mediated by TspB may provide protection against immune responses, which is in accordance with the association of prophage DNA carrying ORF6 with invasive meningococcal strains.
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Registered trials
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