ArticleIntegrative biology : quantitative biosciences from nano to macro2013
Cell mediated contraction in 3D cell-matrix constructs leads to spatially regulated osteogenic differentiation.
Article in Integrative biology : quantitative biosciences from nano to macro, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 29 citations in OpenAlex.
- Bioinspired hydrogel-based regenerative engineering strategies for the structural and functional regeneration of skeletal muscles following volumetric muscle loss.Regenerative biomaterials · 2026Review
- In Vitro Biodegradation and Biocompatibility of Bacterial Nanocellulose-Chitosan-Based Hydrogel Scaffolds for Bone Tissue Engineering.ACS omega · 2025Article
- Mechanical Regulation of Limb Bud Formation.Cells · 2022Review
- Three-Dimensional Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells Promotes Matrix Metallopeptidase 13 (MMP13) Expression in Type I Collagen Hydrogels.International journal of molecular sciences · 2021Article
- Molecular and Mechanical Cues for Somite Periodicity.Frontiers in cell and developmental biology · 2021Review
- Astragalin Promotes Osteoblastic Differentiation in MC3T3-E1 Cells and Bone FormationFrontiers in endocrinology · 2019Article
- Beyond Turing: mechanochemical pattern formation in biological tissues.Biology direct · 2016Article
- Three-dimensional bioprinting of thick vascularized tissues.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- From Skeletal Development to Tissue Engineering: Lessons from the Micromass Assay.Tissue engineering. Part B, Reviews · 2015Review
- Mathematically guided approaches to distinguish models of periodic patterning.Development (Cambridge, England) · 2015Review
- Human embryonic stem cells and macroporous calcium phosphate construct for bone regeneration in cranial defects in rats.Acta biomaterialia · 2014Article
- Three dimensional porous scaffolds derived from collagen, elastin and fibrin proteins orchestrate adipose tissue regeneration.Journal of tissue engineeringArticle
Corrections and comments
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Authors and funding
4 authors at 4 institutions in 2 countries.
Funding
Abstract
During embryonic development, morphogenetic processes give rise to a variety of shapes and patterns that lead to functional tissues and organs. While the impact of chemical signals on these processes is widely studied, the role of physical cues is less understood. The aim of this study was to test the hypothesis that the interplay of cell mediated contraction and mechanical boundary conditions alone can result in spatially regulated differentiation in simple 3D constructs. An experimental model consisting of a 3D cell-gel construct and a finite element (FE) model were used to study the effect of cellular traction exerted by mesenchymal stem cells (MSCs) on an initially homogeneous matrix under inhomogeneous boundary conditions. A robust shape change is observed due to contraction under time-varying mechanical boundary conditions, which is explained by the finite element model. Furthermore, distinct local differences in osteogenic differentiation are observed, with a spatial pattern independent of osteogenic factors in the culture medium. Regions that are predicted to have experienced relatively high shear stress at any time during contraction correlate with the regions of distinct osteogenesis. Taken together, these results support the underlying hypothesis that cellular contractility and mechanical boundary conditions alone can result in spatially regulated differentiation. These results will have important implications for tissue engineering and regeneration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.