ArticleJournal of bone and mineral metabolism2014
Identification of novel regulators of osteoblast matrix mineralization by time series transcriptional profiling.
Article in Journal of bone and mineral metabolism, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- Multiomics profiling of bone in the Dpp3-deficient mice unveils altered protein and lipid composition.Journal of endocrinological investigation · 2026Article
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- Complementary modulation of BMP signaling improves bone healing efficiency.Biomaterials · 2023Article
- PSAT1 positively regulates the osteogenic lineage differentiation of periodontal ligament stem cells through the ATF4/PSAT1/Akt/GSK3β/β-catenin axis.Journal of translational medicine · 2023Article
- Review
- A multi-omics study to characterize the transdifferentiation of human dermal fibroblasts to osteoblast-like cells.Frontiers in molecular biosciences · 2022Article
- Freeze-Dried Secretome (Lyosecretome) from Mesenchymal Stem/Stromal Cells Promotes the Osteoinductive and Osteoconductive Properties of Titanium Cages.International journal of molecular sciences · 2021Article
- A Review of Recent Developments in the Molecular Mechanisms of Bone Healing.International journal of molecular sciences · 2021Review
- Trb3 controls mesenchymal stem cell lineage fate and enhances bone regeneration by scaffold-mediated local gene delivery.Biomaterials · 2021Article
- PHOSPHO1 is a skeletal regulator of insulin resistance and obesity.BMC biology · 2020Article
- Role of the Scavenger Receptor CD36 in Accelerated Diabetic Atherosclerosis.International journal of molecular sciences · 2020Article
- The neuropeptide calcitonin gene-related peptide alpha is essential for bone healing.EBioMedicine · 2020Article
- Lipids in the Bone Marrow: An Evolving Perspective.Cell metabolism · 2020Review
- Raman spectroscopy links differentiating osteoblast matrix signatures to pro-angiogenic potential.Matrix biology plus · 2020Article
- Montmorency tart cherry protects against age-related bone loss in female C57BL/6 mice and demonstrates some anabolic effects.European journal of nutrition · 2019Article
- oxLDL inhibits differentiation of mesenchymal stem cells into osteoblasts via the CD36 mediated suppression of Wnt signaling pathway.Molecular biology reports · 2019Article
- Physiologic and pathologic effects of dietary free fatty acids on cells of the joint.Annals of the New York Academy of Sciences · 2019Review
- Hyperstimulation of CaSR in human MSCs by biomimetic apatite inhibits endochondral ossification via temporal down-regulation of PTH1R.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- FGF-2 promotes osteocyte differentiation through increased E11/podoplanin expression.Journal of cellular physiology · 2018Article
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Authors and funding
4 authors.
Funding
Abstract
Bone mineralization is a carefully orchestrated process, regulated by a number of promoters and inhibitors that function to ensure effective hydroxyapatite formation. Here we sought to identify new regulators of this process through a time series microarray analysis of mineralising primary osteoblast cultures over a 27 day culture period. To our knowledge this is the first microarray study investigating murine calvarial osteoblasts cultured under conditions that permit both physiological extracellular matrix mineralization through the formation of discrete nodules and the terminal differentiation of osteoblasts into osteocytes. RT-qPCR was used to validate and expand the microarray findings. We demonstrate the significant up-regulation of >6,000 genes during the osteoblast mineralization process, the highest-ranked differentially expressed genes of which were those dominated by members of the PPAR-γ signalling pathway, namely Adipoq, Cd36 and Fabp4. Furthermore, we show that the inhibition of this signalling pathway promotes matrix mineralisation in these primary osteoblast cultures. We also identify Cilp, Phex, Trb3, Sox11, and Psat1 as novel regulators of matrix mineralization. Further studies examining the precise function of the identified genes and their interactions will advance our understanding of the mechanisms underpinning biomineralization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.