ArticleProceedings of the National Academy of Sciences of the United States of America2013
A high-throughput screen for quorum-sensing inhibitors that target acyl-homoserine lactone synthases.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
41 citing papers in PubMed, 92 citations in OpenAlex.
- Revealing the secrets of quorum sensing: a comprehensive review from signal mechanisms to practical applications.Science China. Life sciences · 2026Review
- Research Progress on the Quorum Sensing System ofPathogens (Basel, Switzerland) · 2026Review
- Next-generation anti-infective drugs in the post-antibiotic era: focusing on anti-virulence agents targeting the bacterial quorum sensing system.Frontiers in cellular and infection microbiology · 2026Review
- Assembly of functional microbial ecosystems: from molecular circuits to communities.FEMS microbiology reviews · 2024Review
- Characterization of natural product inhibitors of quorum sensing reveals competitive inhibition ofMicrobiology spectrum · 2024Article
- Characterization of natural product inhibitors of quorum sensing inbioRxiv : the preprint server for biology · 2024Article
- Identification of a dCache-type chemoreceptor inFrontiers in microbiology · 2024Article
- Biofilm Lifestyle in Recurrent Urinary Tract Infections.Life (Basel, Switzerland) · 2023Review
- Targeted Anti-Biofilm Therapy: Dissecting Targets in the Biofilm Life Cycle.Pharmaceuticals (Basel, Switzerland) · 2022Review
- Maggot Extract Interrupts Bacterial Biofilm Formation and Maturation in Combination with Antibiotics by Reducing the Expression of Virulence Genes.Life (Basel, Switzerland) · 2022Article
- Pseudomonas aeruginosa Quorum Sensing.Advances in experimental medicine and biology · 2022Article
- Structural insights into acyl-ACP selective recognition by the Aeromonas hydrophila AHL synthase AhyI.BMC microbiology · 2021Article
- Design, synthesis, and evaluation of transition-state analogs as inhibitors of the bacterial quorum sensing autoinducer synthase CepI.Bioorganic & medicinal chemistry letters · 2021Article
- Bacterial quorum sensing quenching activity ofComputational and structural biotechnology journal · 2021Article
- Possible drugs for the treatment of bacterial infections in the future: anti-virulence drugs.The Journal of antibiotics · 2021Review
- Bacterial Biofilm Inhibition: A Focused Review on Recent Therapeutic Strategies for Combating the Biofilm Mediated Infections.Frontiers in microbiology · 2021Review
- Design, Synthesis, and Biochemical Characterization of Non-Native Antagonists of theACS infectious diseases · 2020Article
- Quorum sensing for population-level control of bacteria and potential therapeutic applications.Cellular and molecular life sciences : CMLS · 2020Review
- Phenylacetyl Coenzyme A, Not Phenylacetic Acid, Attenuates CepIR-Regulated Virulence in Burkholderia cenocepacia.Applied and environmental microbiology · 2019Article
- Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Many Proteobacteria use N-acyl-homoserine lactone (acyl-HSL) quorum sensing to control specific genes. Acyl-HSL synthesis requires unique enzymes that use S-adenosyl methionine as an acyl acceptor and amino acid donor. We developed and executed an enzyme-coupled high-throughput cell-free screen to discover acyl-HSL synthase inhibitors. The three strongest inhibitors were equally active against two different acyl-HSL synthases: Burkholderia mallei BmaI1 and Yersinia pestis YspI. Two of these inhibitors showed activity in whole cells. The most potent compound behaves as a noncompetitive inhibitor with a Ki of 0.7 µM and showed activity in a cell-based assay. Quorum-sensing signal synthesis inhibitors will be useful in attempts to understand acyl-HSL synthase catalysis and as a tool in studies of quorum-sensing control of gene expression. Because acyl-HSL quorum-sensing controls virulence of some bacterial pathogens, anti-quorum-sensing chemicals have been sought as potential therapeutic agents. Our screen and identification of acyl-HSL synthase inhibitors serve as a basis for efforts to target quorum-sensing signal synthesis as an antivirulence approach.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.