ArticleAMB Express2013
Characterization of erythrose reductases from filamentous fungi.
Article in AMB Express, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed, 19 citations in OpenAlex.
- Protein rational design and modification of erythrose reductase for the improvement of erythritol production in Yarrowia lipolytica.Bioprocess and biosystems engineering · 2024Article
- Osmolyte Signatures for the Protection ofJournal of fungi (Basel, Switzerland) · 2021Article
- Unexpected roles for ADH1 and SORD in catalyzing the final step of erythritol biosynthesis.The Journal of biological chemistry · 2019Article
- The gold-standard genome ofStudies in mycology · 2018Article
- Erythritol as sweetener-wherefrom and whereto?Applied microbiology and biotechnology · 2018Review
- Characterization of erythrose reductase from Yarrowia lipolytica and its influence on erythritol synthesis.Microbial cell factories · 2017Article
- Genetic engineering of Synechocystis PCC6803 for the photoautotrophic production of the sweetener erythritol.Microbial cell factories · 2016Article
- Erythritol production on wheat straw using Trichoderma reesei.AMB Express · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Proteins with putative erythrose reductase activity have been identified in the filamentous fungi Trichoderma reesei, Aspergillus niger, and Fusarium graminearum by in silico analysis. The proteins found in T. reesei and A. niger had earlier been characterized as glycerol dehydrogenase and aldehyde reductase, respectively. Corresponding genes from all three fungi were cloned, heterologously expressed in Escherichia coli, and purified. Subsequently, they were used to establish optimal enzyme assay conditions. All three enzymes strictly require NADPH as cofactor, whereas with NADH no activity could be observed. The enzymatic characterization of the three enzymes using ten substrates revealed high substrate specificity and activity with D-erythrose and D-threose. The enzymes from T. reesei and A. niger herein showed comparable activities, whereas the one from F. graminearum reached only about a tenth of it for all tested substrates. In order to proof in vivo the proposed enzyme function, we overexpressed the erythrose reductase-encoding gene in T. reesei. An increased production of erythritol by the recombinant strain compared to the parental strain could be detected.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.