ArticleJournal of proteome research2013
Candidate serum biomarkers for early intestinal cancer using 15N metabolic labeling and quantitative proteomics in the ApcMin/+ mouse.
Article in Journal of proteome research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 27 citations in OpenAlex.
- Pan-cancer analysis and oncogenic implications ofJournal of cell communication and signaling · 2025Article
- Ethoxychelerythrine as a potential therapeutic strategy targets PI3K/AKT/mTOR induced mitochondrial apoptosis in the treatment of colorectal cancer.Scientific reports · 2025Article
- Mass Spectrometry Proteomics Characterization of Plasma Biomarkers for Colorectal Cancer Associated With Inflammation.Biomarker insights · 2024Article
- Integrated multi-omic analyses provide insight into colon adenoma susceptibility modulation by the gut microbiota.mSystems · 2023Article
- Screening of Exosome-Derived Proteins and Their Potential as Biomarkers in Diagnostic and Prognostic for Pancreatic Cancer.International journal of molecular sciences · 2023Article
- Downregulation of ITIH3 contributes to cisplatin-based chemotherapy resistance in ovarian carcinoma via the Bcl-2 mediated anti-apoptosis signaling pathway.Oncology letters · 2023Article
- Vitamin D receptor absence does not enhance intestinal tumorigenesis in ApcPirc/+rats.Biology open · 2022Article
- Noninvasive Detection of Colorectal Carcinomas Using Serum Protein Biomarkers.The Journal of surgical research · 2020Article
- Conserved serum protein biomarkers associated with growing early colorectal adenomas.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Plasma Inter-Alpha-Trypsin Inhibitor Heavy Chains H3 and H4 Serve as Novel Diagnostic Biomarkers in Human Colorectal Cancer.Disease markers · 2019Article
- Screening key genes for abdominal aortic aneurysm based on gene expression omnibus dataset.BMC cardiovascular disorders · 2018Article
- A new panel of pancreatic cancer biomarkers discovered using a mass spectrometry-based pipeline.British journal of cancer · 2017Article
- A Timely Shift from Shotgun to Targeted Proteomics and How It Can Be Groundbreaking for Cancer Research.Frontiers in oncology · 2017Review
- Increased variability in Apc(Min)/+ intestinal tissue can be measured with microultrasound.Scientific reports · 2016Article
- Pancreatic preneoplastic lesions plasma signatures and biomarkers based on proteome profiling of mouse models.British journal of cancer · 2015Article
- Understanding the rules of the road: proteomic approaches to interrogate the blood brain barrier.Frontiers in neuroscience · 2015Review
- High abundant protein removal from rodent blood for biomarker discovery.Biochemical and biophysical research communications · 2014Article
- The utility of Apc-mutant rats in modeling human colon cancer.Disease models & mechanisms · 2014Review
- The concentrations of EGFR, LRG1, ITIH4, and F5 in serum correlate with the number of colonic adenomas in ApcPirc/+ rats.Cancer prevention research (Philadelphia, Pa.) · 2014Article
- Proteomics for discovery of candidate colorectal cancer biomarkers.World journal of gastroenterology · 2014Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Current screening procedures for colorectal cancer are imperfect and highly invasive and result in increased mortality rates due to low compliance. The goal of the experiments reported herein is to identify potential blood-based biomarkers indicative of early stage intestinal cancers using the ApcMin/+ mouse model of intestinal cancer as an experimental system. Serum proteins from tumor-bearing ApcMin/+ mice were quantitatively compared to tumor-free Apc+/+ wild-type mice via in anima metabolic labeling with 14N/15N-labeled Spirulina algae and an LTQ Orbitrap mass spectrometer. Out of 1116 total serum proteins quantified, this study identified 40 that were differentially expressed and correlated with the increase in intestinal neoplasms. A subset of these differentially expressed proteins underwent a secondary quantitative screen using selected reaction monitoring-mass spectrometry with stable isotope-labeled peptides. Using both quantitative techniques, we identified MGAM and COL1A1 as downregulated and ITIH3 and F5 as upregulated in serum. All but COL1A1 were similarly differentially expressed in the mRNA of neoplastic colonic tissues of ApcMin/+ mice compared to normal wild-type tissue. These differentially expressed proteins identified in the ApcMin/+ mouse model have provided a set of candidate biomarkers for future validation screens in humans.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.