ArticlePloS one2013
Reproductive effects of two neonicotinoid insecticides on mouse sperm function and early embryonic development in vitro.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it, 115 citations in OpenAlex.
- Effects of Neonicotinoid Pesticide Exposure on Human Health: A Systematic Review.Environmental health perspectives · 2017Pooled it
- Neonicotinoids: insecticides or a threat to public health?Frontiers in public health · 2026Article
- Couples exposure to neonicotinoid insecticides and its influence on in vitro fertilization/intracytoplasmic sperm injection outcomes.Environmental health : a global access science source · 2025Article
- Endocrine Disrupting Agents: Toxicological Aspects on Male Reproduction.Reproductive sciences (Thousand Oaks, Calif.) · 2025Review
- Alpha-Tocopherol Protects Porcine Oocytes from Acetamiprid-Induced Meiotic Defects by Alleviating Oxidative Stress-Mediated Ferroptosis.Antioxidants (Basel, Switzerland) · 2025Article
- Neonicotinoid Residues in Tea Products from China: Contamination Patterns and Implications for Human Exposure.Toxics · 2025Article
- Determination of biochemical and histopathological changes on testicular and epididymis tissues induced by exposure to insecticide Imidacloprid during postnatal development in rats.BMC pharmacology & toxicology · 2023Article
- Insights into Free and Conjugated Forms of Neonicotinoid Insecticides in Human Serum and Their Association with Oxidative Stress.Environment & health (Washington, D.C.) · 2023Article
- Changing on the Concentrations of Neonicotinoids in Rice and Drinking Water through Heat Treatment Process.Molecules (Basel, Switzerland) · 2023Article
- Reproductive Effects ofToxics · 2023Article
- Article
- Article
- The Effect of Increasing the Dose of Acetamiprid and Dichlorvos Pesticides on the Reproductive Performance of Laboratory Mice.Advanced biomedical research · 2022Article
- Associations of neonicotinoids with insulin and glucose homeostasis parameters in US adults: NHANES 2015-2016.Chemosphere · 2022Article
- Exposure to Organophosphate and Neonicotinoid Insecticides and Its Association with Steroid Hormones among Male Reproductive-Age Farmworkers in Northern Thailand.International journal of environmental research and public health · 2021Article
- Pesticides: formulants, distribution pathways and effects on human health - a review.Toxicology reports · 2021Review
- Evaluating imidacloprid exposure among grape field male workers using biological and environmental assessment tools: An exploratory study.International journal of hygiene and environmental health · 2020Article
- A critical review on the potential impacts of neonicotinoid insecticide use: current knowledge of environmental fate, toxicity, and implications for human health.Environmental science. Processes & impacts · 2020Review
- An environmentally realistic pesticide and copper mixture impacts embryonic development and DNA integrity of the Pacific oyster, Crassostrea gigas.Environmental science and pollution research international · 2020Article
- Adverse reproductive effects of S100A9 on bovine sperm and early embryonic development in vitro.PloS one · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Acetamiprid (ACE) and imidacloprid (IMI) are two major members in the family of neonicotinoid pesticides, which are synthesized with a higher selectivity to insects. The present study determined and compared in vitro effects of ACE, IMI and nicotine on mammalian reproduction by using an integrated testing strategy for reproductive toxicology, which covered sperm quality, sperm penetration into oocytes and preimplantation embryonic development. Direct chemical exposure (500 µM or 5 mM) on spermatozoa during capacitation was performed, and in vitro fertilization (IVF) process, zygotes and 2-cell embryos were respectively incubated with chemical-supplemented medium until blastocyst formation to evaluate the reproductive toxicity of these chemicals and monitor the stages mainly affected. Generally, treatment of 500 µM or 5 mM chemicals for 30 min did not change sperm motility and DNA integrity significantly but the fertilization ability in in vitro fertilization (IVF) process, indicating that IVF process could detect and distinguish subtle effect of spermatozoa exposed to different chemicals. Culture experiment in the presence of chemicals in medium showed that fertilization process and zygotes are adversely affected by direct exposure of chemicals (P<0.05), in an order of nicotine>IMI>ACE, whereas developmental progression of 2-cell stage embryos was similar to controls (P>0.05). These findings unveiled the hazardous effects of neonicotinoid pesticides exposure on mammalian sperm fertilization ability as well as embryonic development, raising the concerns that neonicotinoid pesticides may pose reproductive risks on human reproductive health, especially in professional populations.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.