ArticlePloS one2013
Simple system for isothermal DNA amplification coupled to lateral flow detection.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed.
- A low-cost, portable, dual-function readout device for amplification-based point-of-need diagnostics.Applied and environmental microbiology · 2023Article
- Current and Future Advances in the Detection and Surveillance of Biosecurity-Relevant Equine Bacterial Diseases Using Loop-Mediated Isothermal Amplification (LAMP).Animals : an open access journal from MDPI · 2023Review
- An integrated isothermal nucleic acid amplification test to detect HPV16 and HPV18 DNA in resource-limited settings.Science translational medicine · 2023Article
- Rapid isothermal point-of-care test for screening of SARS-CoV-2 (COVID-19).Aspects of molecular medicine · 2023Review
- Paper-based genetic assays with bioconjugated gold nanorods and an automated readout pipeline.Scientific reports · 2022Article
- CRISPR-dCas9 based DNA detection scheme for diagnostics in resource-limited settings.Nanoscale · 2022Article
- Nucleic acid lateral flow assay for simultaneous detection of hygiene indicator bacteria.Analytical and bioanalytical chemistry · 2021Article
- Detection of Streptococcus equi subsp. equi in guttural pouch lavage samples using a loop-mediated isothermal nucleic acid amplification microfluidic device.Journal of veterinary internal medicine · 2021Article
- Review
- Handheld Point-of-Care System for Rapid Detection of SARS-CoV-2 Extracted RNA in under 20 min.ACS central science · 2021Article
- Point-of-care DNA testing by automatically and sequentially performing extraction, amplification and identification in a closed-type cassette.Sensors and actuators. B, Chemical · 2021Article
- Development and Preliminary Application of Multiplex Loop-Mediated Isothermal Amplification Coupled With Lateral Flow Biosensor for Detection ofFrontiers in cellular and infection microbiology · 2021Article
- Integrated nucleic acid testing system to enable TB diagnosis in peripheral settings.Lab on a chip · 2020Article
- Long-term dry storage of enzyme-based reagents for isothermal nucleic acid amplification in a porous matrix for use in point-of-care diagnostic devices.The Analyst · 2020Article
- A mathematical model for a biphasic DNA amplification reaction.Journal of the Royal Society, Interface · 2019Article
- Semiquantitative Nucleic Acid Test with Simultaneous Isotachophoretic Extraction and Amplification.Analytical chemistry · 2018Article
- First characterization of a biphasic, switch-like DNA amplification.The Analyst · 2018Article
- Simple Approaches to Minimally-Instrumented, Microfluidic-Based Point-of-Care Nucleic Acid Amplification Tests.Biosensors · 2018Review
- Miniaturized devices for point of care molecular detection of HIV.Lab on a chip · 2017Review
- Novel approach based on one-tube nested PCR and a lateral flow strip for highly sensitive diagnosis of tuberculous meningitis.PloS one · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
Infectious disease diagnosis in point-of-care settings can be greatly improved through integrated, automated nucleic acid testing devices. We have developed an early prototype for a low-cost system which executes isothermal DNA amplification coupled to nucleic acid lateral flow (NALF) detection in a mesofluidic cartridge attached to a portable instrument. Fluid handling inside the cartridge is facilitated through one-way passive valves, flexible pouches, and electrolysis-driven pumps, which promotes a compact and inexpensive instrument design. The closed-system disposable prevents workspace amplicon contamination. The cartridge design is based on standard scalable manufacturing techniques such as injection molding. Nucleic acid amplification occurs in a two-layer pouch that enables efficient heat transfer. We have demonstrated as proof of principle the amplification and detection of Mycobacterium tuberculosis (M.tb) genomic DNA in the cartridge, using either Loop Mediated Amplification (LAMP) or the Exponential Amplification Reaction (EXPAR), both coupled to NALF detection. We envision that a refined version of this cartridge, including upstream sample preparation coupled to amplification and detection, will enable fully-automated sample-in to answer-out infectious disease diagnosis in primary care settings of low-resource countries with high disease burden.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.