ArticleThe international journal of cardiovascular imaging2013
A novel approach for assessing cardiac fibrosis using label-free second harmonic generation.
Article in The international journal of cardiovascular imaging, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed.
- Classification of collagen remodeling in asthma using second-harmonic generation imaging, supervised machine learning and texture-based analysis.Frontiers in bioinformatics · 2025Article
- Analysis of age-related changes in the left ventricular myocardium with multiphoton microscopy.Biomedical optics express · 2024Article
- Assessment of Ultra-Early-Stage Liver Fibrosis in Human Non-Alcoholic Fatty Liver Disease by Second-Harmonic Generation Microscopy.International journal of molecular sciences · 2022Article
- HDAC Inhibition Reverses Preexisting Diastolic Dysfunction and Blocks Covert Extracellular Matrix Remodeling.Circulation · 2021Article
- Optical coherence tomography and multiphoton microscopy offer new options for the quantification of fibrotic aortic valve disease in ApoEScientific reports · 2021Article
- Characterization of fibrillar collagen isoforms in infarcted mouse hearts using second harmonic generation imaging.Biomedical optics express · 2021Article
- Novel Optics-Based Approaches for Cardiac Electrophysiology: A Review.Frontiers in physiology · 2021Review
- X-ray diffraction and second harmonic imaging reveal new insights into structural alterations caused by pressure-overload in murine hearts.Scientific reports · 2020Article
- Optical coherence tomography imaging of cardiac substrates.Quantitative imaging in medicine and surgery · 2019Review
- Multicontrast endomyocardial imaging by single-channel high-resolution cross-polarization optical coherence tomography.Journal of biophotonics · 2018Article
- Characterizing fibrosis in UUO mice model using multiparametric analysis of phasor distribution from FLIM images.Biomedical optics express · 2016Article
- Quantification and Comparison of Anti-Fibrotic Therapies by Polarized SRM and SHG-Based Morphometry in Rat UUO Model.PloS one · 2016Article
- Quantitative analysis of myocardial tissue with digital autofluorescence microscopy.Journal of pathology informatics · 2016Article
- Label-free, three-dimensional multiphoton microscopy of the connective tissue in the anterior vaginal wall.International urogynecology journal · 2015Article
- Pulse splitter-based nonlinear microscopy for live-cardiomyocyte imaging.Proceedings of SPIE--the International Society for Optical Engineering · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To determine whether second harmonic generation (SHG) can be used as a novel and improved label-free technique for detection of collagen deposition in the heart. To verify whether SHG will allow accurate quantification of altered collagen deposition in diseased hearts following hypertrophic remodelling. Minimally invasive transverse aortic banding (MTAB) of mouse hearts was used to generate a reproducible model of cardiac hypertrophy. Physiological and functional assessment of hypertrophic development was performed using echocardiography and post-mortem analysis of remodelled hearts. Cardiac fibroblasts were isolated from sham-operated and hypertrophied hearts and proliferation rates compared. Multi-photon laser scanning microscopy was used to capture both two-photon excited autofluorescence (TPEF) and SHG images simultaneously in two channels. TPEF images were subtracted from SHG images and the resulting signal intensities from ventricular tissue sections were calculated. Traditional picrosirius red staining was used to verify the suitability of the SHG application. MTAB surgery induced significant hypertrophic remodelling and increased cardiac fibroblast proliferation. A significant increase in the density of collagen fibres between hypertrophic and control tissues (p < 0.05) was evident using SHG. Similar increases and patterns of staining were observed using parallel traditional picrosirius red staining of collagen. Label-free SHG microscopy provides a new alternative method for quantifying collagen deposition in fibrotic hearts.
Indexed as
Identifiers
23921804What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.