ArticleInternational journal of molecular sciences2013
Comparative analysis of osteogenic/chondrogenic differentiation potential in primary limb bud-derived and C3H10T1/2 cell line-based mouse micromass cultures.
Article in International journal of molecular sciences, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Angiogenesis in ossification of the posterior longitudinal ligament: progress from mechanism to targeted intervention.Frontiers in cell and developmental biology · 2026Review
- Parallel Chondrogenesis and Osteogenesis Tissue Morphogenesis in Muscle Tissue via Combinations of TGF-β Supergene Family Members.Cartilage · 2025Article
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- The temporal transcriptomic signature of cartilage formation.Nucleic acids research · 2023Article
- The effects of β-catenin on cardiomyogenesis via Islet-1 and MLIP ubiquitination.Experimental biology and medicine (Maywood, N.J.) · 2022Article
- Enhancement of Osteoblast Function through Extracellular Vesicles Derived from Adipose-Derived Stem Cells.Biomedicines · 2022Article
- A Synchronized Circadian Clock Enhances Early Chondrogenesis.Cartilage · 2021Article
- Article
- Simvastatin Enhances the Chondrogenesis But Not the Osteogenesis of Adipose-Derived Stem Cells in a Hyaluronan Microenvironment.Biomedicines · 2021Article
- Atelocollagen promotes chondrogenic differentiation of human adipose-derived mesenchymal stem cells.Scientific reports · 2020Article
- Heterotopic ossification of tendon and ligament.Journal of cellular and molecular medicine · 2020Review
- Article
- TGFβ1 induces bone formation from BMP9-activated Bone Mesenchymal Stem Cells, with possible involvement of non-canonical pathways.International journal of medical sciences · 2020Article
- Identification of reference genes for quantitative PCR during C3H10T1/2 chondrogenic differentiation.Molecular biology reports · 2019Article
- Alpha-5 Integrin Mediates Simvastatin-Induced Osteogenesis of Bone Marrow Mesenchymal Stem Cells.International journal of molecular sciences · 2019Article
- Identification of satellite cells from anole lizard skeletal muscle and demonstration of expanded musculoskeletal potential.Developmental biology · 2018Article
- Osteogenic Programming of Human Mesenchymal Stem Cells with Highly Efficient Intracellular Delivery of RUNX2.Stem cells translational medicine · 2017Article
- Sika Deer Antler Collagen Type I-Accelerated Osteogenesis in Bone Marrow Mesenchymal Stem Cells via the Smad Pathway.Evidence-based complementary and alternative medicine : eCAM · 2016Article
- Polymodal Transient Receptor Potential Vanilloid (TRPV) Ion Channels in Chondrogenic Cells.International journal of molecular sciences · 2015Article
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5 authors.
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Abstract
Murine micromass models have been extensively applied to study chondrogenesis and osteogenesis to elucidate pathways of endochondral bone formation. Here we provide a detailed comparative analysis of the differentiation potential of micromass cultures established from either BMP-2 overexpressing C3H10T1/2 cells or mouse embryonic limb bud-derived chondroprogenitor cells, using micromass cultures from untransfected C3H10T1/2 cells as controls. Although the BMP-2 overexpressing C3H10T1/2 cells failed to form chondrogenic nodules, cells of both models expressed mRNA transcripts for major cartilage-specific marker genes including Sox9, Acan, Col2a1, Snorc, and Hapln1 at similar temporal sequence, while notable lubricin expression was only detected in primary cultures. Furthermore, mRNA transcripts for markers of osteogenic differentiation including Runx2, Osterix, alkaline phosphatase, osteopontin and osteocalcin were detected in both models, along with matrix calcification. Although the adipogenic lineage-specific marker gene FABP4 was also expressed in micromass cultures, Oil Red O-positive cells along with PPARγ2 transcripts were only detected in C3H10T1/2-derived micromass cultures. Apart from lineage-specific marker genes, pluripotency factors (Nanog and Sox2) were also expressed in these models, reflecting on the presence of various mesenchymal lineages as well as undifferentiated cells. This cellular heterogeneity has to be taken into consideration for the interpretation of data obtained by using these models.
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