ArticleVirology journal2013
Establishment of cell lines with increased susceptibility to EV71/CA16 by stable overexpression of SCARB2.
Article in Virology journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 35 citations in OpenAlex.
- Systemic removal of host restriction factors enables rapid, scalable virus production for cell culture-based vaccine development.Frontiers in bioengineering and biotechnology · 2026Article
- Whole-genome sequencing and phylogenetic analysis of coxsackievirus-A16 strains causing hand, foot and mouth disease (HFMD) in India.Microbial genomics · 2023Article
- Type I Interferon-Induced TMEM106A Blocks Attachment of EV-A71 Virus by Interacting With the Membrane Protein SCARB2.Frontiers in immunology · 2022Article
- Antiviral effects of the petroleum ether extract ofFrontiers in pharmacology · 2022Article
- A hSCARB2-transgenic mouse model for Coxsackievirus A16 pathogenesis.Virology journal · 2021Article
- Myristoylation of EV71 VP4 is Essential for Infectivity and Interaction with Membrane Structure.Virologica Sinica · 2020Article
- Characterization of Plaque Variants and the Involvement of Quasi-Species in a Population of EV-A71.Viruses · 2020Article
- Antiviral Efficacy of Flavonoids against Enterovirus 71 Infection in Vitro and in Newborn Mice.Viruses · 2019Article
- An update on enterovirus 71 infection and interferon type I response.Reviews in medical virology · 2019Review
- Mutations in VP1 and 5'-UTR affect enterovirus 71 virulence.Scientific reports · 2018Article
- Enterovirus 71 infection impairs the reproductive capacity of female mice.Experimental and therapeutic medicine · 2017Article
- Transcriptome analysis reveals dynamic changes in coxsackievirus A16 infected HEK 293T cells.BMC genomics · 2017Article
- A clinically authentic mouse model of enterovirus 71 (EV-A71)-induced neurogenic pulmonary oedema.Scientific reports · 2016Article
- Cooperative effect of the VP1 amino acids 98E, 145A and 169F in the productive infection of mouse cell lines by enterovirus 71 (BS strain).Emerging microbes & infections · 2016Article
- Article
- Tissue Localization of Lymphocystis Disease Virus (LCDV) Receptor-27.8 kDa and Its Expression Kinetics Induced by the Viral Infection in Turbot (Scophthalmus maximus).International journal of molecular sciences · 2015Article
- Viral vaccines and their manufacturing cell substrates: New trends and designs in modern vaccinology.Biotechnology journal · 2015Review
- Article
- Immunogenicity and protective efficacy of an EV71 virus-like particle vaccine against lethal challenge in newborn mice.Human vaccines & immunotherapeutics · 2015Article
- Article
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Authors and funding
12 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHuman enterovirus type 71 (EV71) and Coxsackievirus A group type 16 (CA16) belong to human Enterovirus species A of the family Picornaviridae. These viruses are recognized as the major pathogens responsible for epidemics of hand-foot-mouth disease (HFMD), which presents with fever and vesicular eruptions of palms, soles of the feet or mouth. Human scavenger receptor class B, member 2 (SCARB2) has been identified as the receptor for both EV71 and CA16, as overexpression of SCARB2 in cells can enhance virus replication significantly.
methodsIn this study, we used a lentivirus packaging vector to transduce the SCARB2 gene into human embryonic kidney cells (293), human rhabdomyosarcoma cells (RD) and African green monkey kidney cells (Vero) to create stable expression lines. Expression of SCARB2 in the resulting three transgenic cell lines was confirmed by real-time RT-PCR, immunofluorescence and flow cytometry.
resultsLevels of SCARB2 mRNA determined by real-time RT-PCR in 293-SCARB2 (293S) or RD-SCARB2 (RDS) transgenic cell lines were approximately 2 × 10(2) times higher than those in 293 and RD cells, respectively, and three times higher in Vero-SCARB2 (VeroS) than in Vero cells. Furthermore, EV71 and CA16 virus titers in 293S and RDS cells were 10(2)-10(3)-fold higher (detected in RD cell) than those in the parental cells, and a 10-fold higher titer of EV71 was achieved in VeroS cells compared with that in Vero cells.
conclusionsWe established for the first time three cell lines stably overexpressing SCARB2, which showed drastic increases in susceptibility to EV71/CA16 infection. These optimal cell lines may be utilized to develop inactivated vaccines for EV71/CA16 and facilitate rapid detection and isolation of HFMD pathogens or other Enterovirus serotypes. Furthermore, these stable cell lines also can serve as tools to facilitate drug screenings as well as molecular studies on virus-host interactions and pathogenesis of causative agents for HFMD.
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