ArticleJournal of experimental botany2013
OsAP65, a rice aspartic protease, is essential for male fertility and plays a role in pollen germination and pollen tube growth.
Article in Journal of experimental botany, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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38 citing papers in PubMed, 75 citations in OpenAlex.
- Review
- The Pentatricopeptide Repeat Protein OsPPR7 Confers Cold Tolerance at the Booting Stage in Rice by Regulating ABA and ROS Homeostasis.Rice (New York, N.Y.) · 2026Article
- Establishment of fluorescent protein-tagged lines for investigating dynamic localization of organelle and other cell structures in rice pollen tube.Plant methods · 2026Article
- Structural defects in male meiotic cell division lead to reproductive genome instability and disturbed microsporogenesis in banana (Frontiers in plant science · 2026Article
- Enhanced lignin and cellulose metabolism promote cell wall synthesis and growth of wild soybean HRA under alkali stress.Annals of botany · 2025Article
- The OsAP4-OsCATA/OsCATC Regulatory Module Orchestrates Drought Stress Adaptation in Rice Seedlings Through ROS Scavenging.Plants (Basel, Switzerland) · 2025Article
- Genome-wide identification and expression patterns of the aspartic protease gene family in Epimedium pubescens.BMC genomics · 2025Article
- Regulatory analysis of root architectural and anatomical adaptation to nitrate and ammonium inFrontiers in plant science · 2025Article
- Functional Characterization ofJournal of fungi (Basel, Switzerland) · 2024Article
- qCTB7 positively regulates cold tolerance at booting stage in rice.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Two aspartic proteases, BnaAP36s and BnaAP39s, regulate pollen tube guidance inMolecular breeding : new strategies in plant improvement · 2023Article
- Genetic characterization and fine mapping ofMolecular breeding : new strategies in plant improvement · 2022Article
- The grapevine aspartic protease gene family: characterization and expression modulation in response to Plasmopara viticola.Journal of plant research · 2022Article
- Genome-Wide Analyses of Aspartic Proteases on Potato Genome (Plants (Basel, Switzerland) · 2022Article
- Heat-induced proteomic changes in anthers of contrasting rice genotypes under variable stress regimes.Frontiers in plant science · 2022Article
- Proteolysis in Reproduction: Lessons From Gene-Modified Organism Studies.Frontiers in endocrinology · 2022Review
- Transcriptional and post-transcriptional regulation of ethylene biosynthesis by exogenous acetylsalicylic acid in kiwifruit.Horticulture research · 2022Article
- Multi-omics analysis the differences of VOCs terpenoid synthesis pathway in maintaining obligate mutualism betweenFrontiers in plant science · 2022Article
- Defense and Offense Strategies: The Role of Aspartic Proteases in Plant-Pathogen Interactions.Biology · 2021Review
- Genome-wide identification, evolution and expression analysis of the aspartic protease gene family during rapid growth of moso bamboo (Phyllostachys edulis) shoots.BMC genomics · 2021Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Aspartic proteases (APs) comprise a large proteolytic enzyme family widely distributed in animals, microbes, viruses, and plants. The rice genome encodes 96 APs, of which only a few have been functionally characterized. Here, the identification and characterization of a novel AP gene, OsAP65, which plays an indispensable role in pollen tube growth in rice, is reported. The T-DNA insertion line of OsAP65 caused severe segregation distortion. In the progeny derived from an individual heterozygous for the T-DNA insertion, the wild type and T-DNA-carrying heterozygote segregated at a ratio close to 1:1, while homozygotes of disrupted OsAP65 (OsAP65-/-) were not recovered. Reciprocal crosses between heterozygotes and wild-type plants demonstrated that the mutant alleles could not be transmitted through the male gamete. Examination of the anthers from heterozygous plants revealed that the mutant pollen matured normally, but did not germinate or elongate. OsAP65 was expressed in various tissues and the transcript level in heterozygous plants was about half of the amount measured in the wild-type plants. The subcellular localization showed that OsAP65 is a pre-vacuolar compartment (PVC) protein. These results indicated that OsAP65 was essential for rice pollen germination and tube growth.
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