ArticleThe Journal of cell biology2013
Microtubule severing by the katanin complex is activated by PPFR-1-dependent MEI-1 dephosphorylation.
Article in The Journal of cell biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 23 citations in OpenAlex.
- Research Progress on Regulatory Mechanisms of Conical Cell Morphogenesis inPlants (Basel, Switzerland) · 2026Review
- N-terminal phosphorylation inhibits Arabidopsis katanin and affects vegetative and reproductive development in opposite ways.bioRxiv : the preprint server for biology · 2026Article
- Microtubule-binding domains in Katanin p80 subunit are essential for severing activity in C. elegans.The Journal of cell biology · 2024Article
- The katanin A-subunits KATNA1 and KATNAL1 act co-operatively in mammalian meiosis and spermiogenesis to achieve male fertility.Development (Cambridge, England) · 2023Article
- Nuclear-enriched protein phosphatase 4 ensures outer kinetochore assembly prior to nuclear dissolution.The Journal of cell biology · 2023Article
- STRIPAK regulation of katanin microtubule severing in the Caenorhabditis elegans embryo.Genetics · 2022Article
- The Mammalian Family of Katanin Microtubule-Severing Enzymes.Frontiers in cell and developmental biology · 2021Review
- C. elegans CLASP/CLS-2 negatively regulates membrane ingression throughout the oocyte cortex and is required for polar body extrusion.PLoS genetics · 2020Article
- Phosphorylation of the microtubule-severing AAA+ enzyme Katanin regulates C. elegans embryo development.The Journal of cell biology · 2020Article
- Phosphatases in Mitosis: Roles and Regulation.Biomolecules · 2019Review
- Creation and testing of a new, local microtubule-disruption tool based on the microtubule-severing enzyme, katanin p60.Cytoskeleton (Hoboken, N.J.) · 2018Article
- Regulation of the MEI-1/MEI-2 Microtubule-Severing Katanin Complex in Early Caenorhabditis elegans Development.G3 (Bethesda, Md.) · 2016Article
- Oocyte Meiotic Spindle Assembly and Function.Current topics in developmental biology · 2016Review
- Katanin maintains meiotic metaphase chromosome alignment and spindle structure in vivo and has multiple effects on microtubules in vitro.Molecular biology of the cell · 2014Article
- Caenorhabditis elegans oocyte meiotic spindle pole assembly requires microtubule severing and the calponin homology domain protein ASPM-1.Molecular biology of the cell · 2014Article
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Authors and funding
8 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Katanin is an evolutionarily conserved microtubule (MT)-severing complex implicated in multiple aspects of MT dynamics. In Caenorhabditis elegans, the katanin homologue MEI-1 is required for meiosis, but must be inactivated before mitosis. Here we show that PPFR-1, a regulatory subunit of a trimeric protein phosphatase 4 complex, enhanced katanin MT-severing activity during C. elegans meiosis. Loss of ppfr-1, similarly to the inactivation of MT severing, caused a specific defect in meiosis II spindle disassembly. We show that a fraction of PPFR-1 was degraded after meiosis, contributing to katanin inactivation. PPFR-1 interacted with MEL-26, the substrate recognition subunit of the CUL-3 RING E3 ligase (CRL3(MEL-26)), which also targeted MEI-1 for post-meiotic degradation. Reversible protein phosphorylation of MEI-1 may ensure temporal activation of the katanin complex during meiosis, whereas CRL3(MEL-26)-mediated degradation of both MEI-1 and its activator PPFR-1 ensure efficient katanin inactivation in the transition to mitosis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.