ArticleProceedings of the National Academy of Sciences of the United States of America2013
An allele of the crm gene blocks cyanobacterial circadian rhythms.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 26 citations in OpenAlex.
- Circadian regulation of metabolism across photosynthetic organisms.The Plant journal : for cell and molecular biology · 2023Review
- Comparative Genomics of Synechococcus elongatus Explains the Phenotypic Diversity of the Strains.mBio · 2022Article
- Reconstitution of an intact clock reveals mechanisms of circadian timekeeping.Science (New York, N.Y.) · 2021Article
- Genome-wide fitness assessment during diurnal growth reveals an expanded role of the cyanobacterial circadian clock protein KaiA.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Minimal tool set for a prokaryotic circadian clock.BMC evolutionary biology · 2017Article
- Article
- Redox crisis underlies conditional light-dark lethality in cyanobacterial mutants that lack the circadian regulator, RpaA.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- A Combined Computational and Genetic Approach Uncovers Network Interactions of the Cyanobacterial Circadian Clock.Journal of bacteriology · 2016Article
- Circadian Rhythms in Cyanobacteria.Microbiology and molecular biology reviews : MMBR · 2015Review
- The circadian oscillator in Synechococcus elongatus controls metabolite partitioning during diurnal growth.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Cross-talk and regulatory interactions between the essential response regulator RpaB and cyanobacterial circadian clock output.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Giving Time Purpose: The Synechococcus elongatus Clock in a Broader Network Context.Annual review of genetics · 2015Review
- Single mutations in sasA enable a simpler ΔcikA gene network architecture with equivalent circadian properties.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Metabolic compensation and circadian resilience in prokaryotic cyanobacteria.Annual review of biochemistry · 2014Review
- Active output state of the Synechococcus Kai circadian oscillator.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The SasA-RpaA two-component system constitutes a key output pathway of the cyanobacterial Kai circadian oscillator. To date, rhythm of phycobilisome associated (rpaA) is the only gene other than kaiA, kaiB, and kaiC, which encode the oscillator itself, whose mutation causes completely arrhythmic gene expression. Here we report a unique transposon insertion allele in a small ORF located immediately upstream of rpaA in Synechococcus elongatus PCC 7942 termed crm (for circadian rhythmicity modulator), which results in arrhythmic promoter activity but does not affect steady-state levels of RpaA. The crm ORF complements the defect when expressed in trans, but only if it can be translated, suggesting that crm encodes a small protein. The crm1 insertion allele phenotypes are distinct from those of an rpaA null; crm1 mutants are able to grow in a light:dark cycle and have no detectable oscillations of KaiC phosphorylation, whereas low-amplitude KaiC phosphorylation rhythms persist in the absence of RpaA. Levels of phosphorylated RpaA in vivo measured over time are significantly altered compared with WT in the crm1 mutant as well as in the absence of KaiC. Taken together, these results are consistent with the hypothesis that the Crm polypeptide modulates a circadian-specific activity of RpaA.
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