ArticleInfection control and hospital epidemiology2013
Detection of mixed populations of Clostridium difficile from symptomatic patients using capillary-based polymerase chain reaction ribotyping.
Article in Infection control and hospital epidemiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Antimicrobial susceptibility and ribotypes of Clostridium difficile isolates from a Phase 2 clinical trial of ridinilazole (SMT19969) and vancomycin.The Journal of antimicrobial chemotherapy · 2018Trial
- Multi-strain Clostridioides difficile infection: Increased detection and clinical implications.The Journal of infection · 2026Article
- A molecular epidemiological and transmission analysis of Clostridioides difficile using draft whole-genome sequencing in a single hospital.BMC infectious diseases · 2024Article
- A US-based national surveillance study for the susceptibility and epidemiology ofAntimicrobial agents and chemotherapy · 2023Article
- Longitudinal genomic surveillance of carriage and transmission of Clostridioides difficile in an intensive care unit.Nature medicine · 2023Article
- A local-scale One Health genomic surveillance ofMicrobial genomics · 2023Article
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- Laboratory-Based Surveillance of Clostridium difficile Infection in Australian Health Care and Community Settings, 2013 to 2018.Journal of clinical microbiology · 2020Article
- A Review of Mixed StrainFrontiers in microbiology · 2019Review
- Phenotypic Characterization of Non-toxigenicFrontiers in microbiology · 2019Article
- Presence of multiple Clostridium difficile strains at primary infection is associated with development of recurrent disease.Anaerobe · 2018Article
- Risk Factors for Clostridium difficile Isolation in Inflammatory Bowel Disease: A Prospective Study.Digestive diseases and sciences · 2018Article
- Prevalence of binary toxin positive Clostridium difficile in diarrhoeal humans in the absence of epidemic ribotype 027.PloS one · 2017Article
- Role of Coinfecting Strains in Recurrent Clostridium difficile Infection.Infection control and hospital epidemiology · 2016Article
- Comparative genomic analysis of toxin-negative strains of Clostridium difficile from humans and animals with symptoms of gastrointestinal disease.BMC microbiology · 2016Article
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- Asymptomatic Clostridium difficile colonization: epidemiology and clinical implications.BMC infectious diseases · 2015Review
- Evaluation of portability and cost of a fluorescent PCR ribotyping protocol for Clostridium difficile epidemiology.Journal of clinical microbiology · 2015Article
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Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
objectiveTo investigate the simultaneous occurrence of more than 1 Clostridium difficile ribotype in patients' stool samples at the time of diagnostic testing.
methodsStool samples submitted for diagnostic testing for the presence of toxigenic C. difficile were obtained for 102 unique patients. A total of 95 single colonies of C. difficile per stool sample were isolated on selective media, subcultured alongside negative (uninoculated) controls, and polymerase chain reaction (PCR) ribotyped using capillary gel electrophoresis.
resultsCapillary-based PCR ribotyping was successful for 9,335 C. difficile isolates, yielding a median of 93 characterized isolates per stool sample (range, 69-95). More than 1 C. difficile ribotype was present in 16 of 102 (16%) C. difficile infection (CDI) cases; 2 of the 16 mixtures were composed of at least 3 ribotypes, while the remaining 14 were composed of at least 2.
conclusionsDeep sampling of patient stool samples coupled with capillary-based PCR ribotyping identified a high rate of mixed CDI cases compared with previous estimates. Studies seeking to quantify the clinical significance of particular C. difficile ribotypes should account for mixed cases of disease.
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