ReviewBiotechnology advances2013
CHO microRNA engineering is growing up: recent successes and future challenges.
Review in Biotechnology advances, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 90 citations in OpenAlex.
- Evaluation of precursor MicroRNA (pre-miRNA) as a powerful tool for robust CHO production cell line platform development.Bioprocess and biosystems engineering · 2025Article
- A CRISPR-HITI strategy approach to improve CHO cell viability by modifying the 3'UTR of Caspase 8 Associated Protein 2.Molecular biology research communications · 2025Article
- Next-generation sequencing: A powerful multi-purpose tool in cell line development for biologics production.Computational and structural biotechnology journal · 2025Review
- Conditional Knockdown of Endogenous MicroRNAs in CHO Cells Using TET-ON-SanDI Sponge Vectors.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Review
- Enhancing protein production and growth in chinese hamster ovary cells through miR-107 overexpression.AMB Express · 2024Article
- CRISPR Deletion of miR-27 Impacts Recombinant Protein Production in CHO Cells.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Genome-Wide High-Throughput RNAi Screening for Identification of Genes Involved in Protein Production.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Review
- Overexpression of miR-32 in Chinese hamster ovary cells increases production of Fc-fusion protein.AMB Express · 2023Article
- Recent developments in miRNA based recombinant protein expression in CHO.Biotechnology letters · 2022Review
- The Effect of microRNA on the Production of Recombinant Protein in CHO Cells and its Mechanism.Frontiers in bioengineering and biotechnology · 2022Review
- High Throughput miRNA Screening Identifies miR-574-3p Hyperproductive Effect in CHO Cells.Biomolecules · 2021Article
- Elucidating the impact of cottonseed hydrolysates on CHO cell culture performance through transcriptomic analysis.Applied microbiology and biotechnology · 2021Article
- Expression vector cassette engineering for recombinant therapeutic production in mammalian cell systems.Applied microbiology and biotechnology · 2020Review
- Oxidative stress-alleviating strategies to improve recombinant protein production in CHO cells.Biotechnology and bioengineering · 2020Review
- Improved protein expression in HEK293 cells by over-expressing miR-22 and knocking-out its target gene, HIPK1.New biotechnology · 2020Article
- Enhanced transient expression of an anti-CD52 monoclonal antibody in CHO cells through utilization of miRNA sponge technology.Research in pharmaceutical sciences · 2019Article
- A reference genome of the Chinese hamster based on a hybrid assembly strategy.Biotechnology and bioengineering · 2018Article
- Identifying HIPK1 as Target of miR-22-3p Enhancing Recombinant Protein Production From HEK 293 Cell by Using Microarray and HTP siRNA Screen.Biotechnology journal · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 5 institutions in 4 countries.
Funding
Abstract
microRNAs with their ability to regulate complex pathways that control cellular behavior and phenotype have been proposed as potential targets for cell engineering in the context of optimization of biopharmaceutical production cell lines, specifically of Chinese Hamster Ovary cells. However, until recently, research was limited by a lack of genomic sequence information on this industrially important cell line. With the publication of the genomic sequence and other relevant data sets for CHO cells since 2011, the doors have been opened for an improved understanding of CHO cell physiology and for the development of the necessary tools for novel engineering strategies. In the present review we discuss both knowledge on the regulatory mechanisms of microRNAs obtained from other biological models and proof of concepts already performed on CHO cells, thus providing an outlook of potential applications of microRNA engineering in production cell lines.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.