ArticleThe Journal of allergy and clinical immunology2013
The novel structure of the cockroach allergen Bla g 1 has implications for allergenicity and exposure assessment.
Article in The Journal of allergy and clinical immunology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 71 citations in OpenAlex.
- Epitope-Specific Antibodies in Allergic Disease and Clinical Tolerance.Immunological reviews · 2025Review
- Airborne exposure-induced occupational type I allergy to Tenebrio molitor: Case report and studies on immunological reactivity.Allergologie select · 2025Article
- Indoor allergen exposure and its association to upper respiratory infections and pulmonary outcomes among children with asthma.The Journal of allergy and clinical immunology · 2024Article
- Regulation of Airway Epithelial-Derived Alarmins in Asthma: Perspectives for Therapeutic Targets.Biomedicines · 2024Review
- Production and Release of Proinflammatory Mediators by the Cockroach Allergen Bla g 1 via a Shared Membrane-Destabilization Mechanism.Biochemistry · 2024Article
- Cockroach allergy: Understanding complex immune responses to develop novel therapies.Molecular immunology · 2023Article
- The Identification of Potential Immunogenic Antigens in Particular Active Developmental Stages of the Rice Weevil (International journal of environmental research and public health · 2023Article
- Ampicillin treated German cockroach extract leads to reduced inflammation in human lung cells and a mouse model of Asthma.Parasites, hosts and diseases · 2023Article
- In Silico Comparative Exploration of Allergens ofMolecules (Basel, Switzerland) · 2022Article
- Systematic analysis of membrane contact sites ineLife · 2022Article
- Transcriptome Analysis Reveals Early Hemocyte Responses upon In Vivo Stimulation with LPS in the Stick InsectInsects · 2022Article
- Allergens and their associated small molecule ligands-their dual role in sensitization.Allergy · 2021Review
- The mosquito protein AEG12 displays both cytolytic and antiviral properties via a common lipid transfer mechanism.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Removal and Replacement of Endogenous Ligands from Lipid-Bound Proteins and Allergens.Journal of visualized experiments : JoVE · 2021Article
- Editorial: Activation of Innate Immunity by Allergens and Allergenic Sources.Frontiers in allergy · 2021Article
- Abundance and Stability as Common Properties of Allergens.Frontiers in allergy · 2021Review
- Reduced production of the major allergens Bla g 1 and Bla g 2 in Blattella germanica after antibiotic treatment.PloS one · 2021Article
- The allergenic activity and clinical impact of individual IgE-antibody binding molecules from indoor allergen sources.The World Allergy Organization journal · 2020Review
- Hydrophobic ligands influence the structure, stability, and processing of the major cockroach allergen Bla g 1.Scientific reports · 2019Article
- Cockroach allergen component analysis of children with or without asthma and rhinitis in an inner-city birth cohort.The Journal of allergy and clinical immunology · 2019Observational
Corrections and comments
- Erratum issued
Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundSensitization to cockroach allergens is a major risk factor for asthma. The cockroach allergen Bla g 1 has multiple repeats of approximately 100 amino acids, but the fold of the protein and its biological function are unknown.
objectiveWe sought to determine the structure of Bla g 1, investigate the implications for allergic disease, and standardize cockroach exposure assays.
methodsnBla g 1 and recombinant constructs were compared by using ELISA with specific murine IgG and human IgE. The structure of Bla g 1 was determined by x-ray crystallography. Mass spectrometry and nuclear magnetic resonance spectroscopy were used to examine the ligand-binding properties of the allergen.
resultsThe structure of an rBla g 1 construct with comparable IgE and IgG reactivity to the natural allergen was solved by x-ray crystallography. The Bla g 1 repeat forms a novel fold with 6 helices. Two repeats encapsulate a large and nearly spherical hydrophobic cavity, defining the basic structural unit. Lipids in the cavity varied depending on the allergen origin. Palmitic, oleic, and stearic acids were associated with nBla g 1 from cockroach frass. One unit of Bla g 1 was equivalent to 104 ng of allergen.
conclusionsBla g 1 has a novel fold with a capacity to bind various lipids, which suggests a digestive function associated with nonspecific transport of lipid molecules in cockroaches. Defining the basic structural unit of Bla g 1 facilitates the standardization of assays in absolute units for the assessment of environmental allergen exposure.
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