ArticleCellular and molecular bioengineering2013
Modulation of Nuclear Shape by Substrate Rigidity.
Article in Cellular and molecular bioengineering, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 91 papers, 1 of them a synthesis that pooled it.
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Who cites it
91 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Mechanosensing by the nucleus: From pathways to scaling relationships.The Journal of cell biology · 2017Pooled it
- Integration of nuclear mechanosensing with integrin-extracellular matrix adhesions.Nucleus (Austin, Tex.) · 2026Review
- SAF-A/HNRNPU regulates euchromatin dynamics and is required for nuclear envelope integrity.bioRxiv : the preprint server for biology · 2026Article
- Substrate curvature and stiffness equivalence in governing cell mechanosensing.Nature communications · 2026Article
- Matrix stiffness induces midnolin-dependent lamin B1 degradation to control myoblast differentiation.EMBO reports · 2026Article
- Mesenchymal stem cells differentiate dynamic from static load through variable regulation of the Hippo pathway.Journal of biomechanics · 2026Article
- Nuclear Mechanics and Nuclear Mechanotransduction in Cancer Cell Migration and Invasion.Biomolecules · 2026Review
- Mechanoepigenetic Targeting of Histone Methyltransferase EZH2 Increases Potential of Scalable Manufacturing in Human Derived Primary Mesenchymal Stromal Cells.bioRxiv : the preprint server for biology · 2025Article
- A multiscale theory for mesenchymal cell migration in straight or curved channel confinement.Biophysical journal · 2025Article
- Depletion of SUN1/2 induces heterochromatin accrual in mesenchymal stem cells during adipogenesis.Communications biology · 2025Article
- Review
- Divergent Contribution of Cytoplasmic Actins to Nuclear Structure of Lung Cancer Cells.International journal of molecular sciences · 2024Article
- Article
- Sensing the squeeze: nuclear mechanotransduction in health and disease.Nucleus (Austin, Tex.) · 2024Review
- Matrix stiffness drives drop like nuclear deformation and lamin A/C tension-dependent YAP nuclear localization.Nature communications · 2024Article
- Genetic variation drives cancer cell adaptation to ECM stiffness.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Ultrasound-triggered three dimensional hyaluronic acid hydrogel promotes in vitro and in vivo reprogramming into induced pluripotent stem cells.Bioactive materials · 2024Article
- A homeostatic role of nucleus-actin filament coupling in the regulation of cellular traction forces in fibroblasts.Biomechanics and modeling in mechanobiology · 2024Article
- Nucleocytoplasmic transport rates are regulated by cellular processes that modulate GTP availability.The Journal of cell biology · 2024Article
- Data-Driven and Cell-Specific Determination of Nuclei-Associated Actin Structure.Small structures · 2024Article
31 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
The nucleus is mechanically coupled to the three cytoskeletal elements in the cell via linkages maintained by the LINC complex (for Linker of Nucleoskeleton to Cyto-skeleton). It has been shown that mechanical forces from the extracellular matrix (ECM) can be transmitted through the cytoskeleton to the nuclear surface. Here we quantified nuclear shape in NIH 3T3 fibroblasts on polyacrylamide gels with a controlled degree of cross-linking. On soft substrates with a Young's modulus of 0.4 kPa, the nucleus appeared rounded in its vertical cross-section, while on stiff substrates (308 kPa), the nucleus appears more flattened. Over-expression of dominant negative Klarsicht ANC-1 Syne Homology (KASH) domains, which disrupts the LINC complex, eliminated the sensitivity of nuclear shape to substrate rigidity; myosin inhibition had similar effects. GFP-KASH4 over-expression altered the rigidity dependence of cell motility and cell spreading. Taken together, our results suggest that nuclear shape is modulated by substrate rigidity-induced changes in actomyosin tension, and that a mechanically integrated nucleus-cytoskeleton is required for rigidity sensing. These results are significant because they suggest that substrate rigidity can potentially control nuclear function and hence cell function.
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Registered trials
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